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Bibliographic Details
Main Authors: Schauberger, Clemens, Middelboe, Mathias, Glud, Ronnie N
Format: Dataset Open Access
Language:en
Published: PANGAEA 2024
Subjects:
Abundance; Bacteria; CTD/Rosette; CTD-RO; Date/Time of event; Deep sea; DEPTH, water; Elevation of event; Event label; Flow cytometry system, Becton Dickinson, FACSCanto II; Hadal trench; Latitude of event; Longitude of event; Microbial abundance, cells; Microbial abundance, standard deviation; prokaryote; SO261; SO261_108-1; SO261_110-1; SO261_19-1; SO261_34-1; SO261_47-1; SO261_62-1; SO261_7-2; SO261_74-1; SO261_80-1; SO261_83-1; Sonne_2; Station label; Viral abundance; Viral abundance, standard deviation; virus-like particles; Water sample; WS
Online Access:https://doi.org/10.1594/PANGAEA.965921
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Table of Contents:
  • Water samples were collected using 7.5-Liter Niskin bottles and fixed with 25% electron microscopy graded glutaraldehyde (1% final concentration) and stored at -80°C until quantification by flow cytometry. Samples were measured in triplicates using a BD FACSCanto™ II flow cytometer, after staining with SYBR Green I (Brussaard 2004). The flow rate was 5–7 μl/min, as determined by BD Trucount™ Beads. The laser settings and gating examples can be found in the Supporting Information of Schauberger et al. (2021).

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