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| Format: | Dataset Open Access |
| Language: | en |
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PANGAEA
2024
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| Online Access: | https://doi.org/10.1594/PANGAEA.972789 |
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| _version_ | 1867171045363941376 |
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| author | Trautmann, Moritz Bartsch, Inka Bligh, Margot Buck-Wiese, Hagen Hehemann, Jan-Hendrik Niedzwiedz, Sarina Plag, Niklas Shan, Tifeng Bischof, Kai Diehl, Nora |
| author_facet | Trautmann, Moritz Bartsch, Inka Bligh, Margot Buck-Wiese, Hagen Hehemann, Jan-Hendrik Niedzwiedz, Sarina Plag, Niklas Shan, Tifeng Bischof, Kai Diehl, Nora |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Global warming affects the Arctic much faster than the rest of the globe, with the highest amplitude of temperature rise occurring during the Polar Night. To determine the stress resistance of the ecosystem-engineering kelp Laminaria digitata against Arctic winter warming, non-meristematic discs of adult sporophytes from Porsangerfjorden (Finnmark, Norway) were kept in total darkness at 0°C and 5°C over a period of three months. The maximum photosynthetic quantum yield of photosystem II (Fv/Fm; Imaging-PAM, Walz GmbH Mess- und Regeltechnik, Effeltrich, Germany) was monitored once a week. For monitoring of potential growth, the size (area) of the algal discs was photographed every two weeks and analyzed with ImageJ (Version 1.52a). Every four weeks, subsamples for monitoring the dry weight and for the biochemical analyses were taken. Laminarin content was determined following via enzymatic digestion. C:N ratio was analyzed with an Elementar Analyzer. Mannitol concentration was analyzed using a HPLC. Absolut pigment concentrations were also analyzed using a HPLC and pool sizes, the de-epoxidation state of the xanthophyll cycle (DPS), and the ratios calculated afterwards. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_972789 |
| institution | PANGAEA |
| language | en |
| publishDate | 2024 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Impact of climate change on the kelp Laminaria digitata: Biochemical and physiological raw data of a long-term experiment simulating Artic winter warming Trautmann, Moritz Bartsch, Inka Bligh, Margot Buck-Wiese, Hagen Hehemann, Jan-Hendrik Niedzwiedz, Sarina Plag, Niklas Shan, Tifeng Bischof, Kai Diehl, Nora Algae Grabber via boat; Antheraxanthin, per dry mass; Arctic Amplification; Arctic Biodiversity & Livelihoods; Area; beta-Carotene, per dry mass; Biomass, dry mass; C:N; Carbon, total, per dry mass; Carbon/Nitrogen ratio; Chlorophyll a, per dry mass; Chlorophyll c2, per dry mass; Chlorophyll Fluorescence System (I-PAM), Walz, Imaging-PAM fluorometer; CHSN Elemental Analyser, Elementar Analysen systeme GmbH, Germany, vario EL III; Date/time end, experiment; Date/time start, experiment; Enzymatic digestion; FACE-IT; Family; Fucoxanthin, per dry mass; Fv/Fm; High performance liquid chromatography (HPLC), Agilent Technologies, 1200 Series; coupled with Aminex Fast Carbohydrate analysis pillar HPAP (100 х 7,8 mm, 9 μ), BioRad front pillar: Phenomenex, Carbo-Pb2+ 4 x 3.00 mm i.d; High performance liquid chromatography (HPLC), VWR Hitachi International, LaChromElite® system, L-2200 autosampler [cooled], DA-detector L-2450, [Pillar: Spherisorb® ODS-2 (250 × 4.6 mm, 5 μm), Waters]; ImageJ (Version 1.52a); laminarin; Laminarin, per dry mass; Location; Mannitol; Mannitol, per dry mass; Maximum quantum yield of photosystem II; Nitrogen, total, per dry mass; Pigments; polar night; Porsangerfjorden_1; Porsangerfjorden_2; Porsangerfjorden_3; Porsangerfjorden, Norway; Precision Balance, Sartorius, MC 21S; Replicate; Species; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Treatment: light:dark cycle; Treatment: temperature; Type of study; Violaxanthin, per dry mass; Zeaxanthin, per dry mass Global warming affects the Arctic much faster than the rest of the globe, with the highest amplitude of temperature rise occurring during the Polar Night. To determine the stress resistance of the ecosystem-engineering kelp Laminaria digitata against Arctic winter warming, non-meristematic discs of adult sporophytes from Porsangerfjorden (Finnmark, Norway) were kept in total darkness at 0°C and 5°C over a period of three months. The maximum photosynthetic quantum yield of photosystem II (Fv/Fm; Imaging-PAM, Walz GmbH Mess- und Regeltechnik, Effeltrich, Germany) was monitored once a week. For monitoring of potential growth, the size (area) of the algal discs was photographed every two weeks and analyzed with ImageJ (Version 1.52a). Every four weeks, subsamples for monitoring the dry weight and for the biochemical analyses were taken. Laminarin content was determined following via enzymatic digestion. C:N ratio was analyzed with an Elementar Analyzer. Mannitol concentration was analyzed using a HPLC. Absolut pigment concentrations were also analyzed using a HPLC and pool sizes, the de-epoxidation state of the xanthophyll cycle (DPS), and the ratios calculated afterwards. |
| title | Impact of climate change on the kelp Laminaria digitata: Biochemical and physiological raw data of a long-term experiment simulating Artic winter warming |
| topic | Algae Grabber via boat; Antheraxanthin, per dry mass; Arctic Amplification; Arctic Biodiversity & Livelihoods; Area; beta-Carotene, per dry mass; Biomass, dry mass; C:N; Carbon, total, per dry mass; Carbon/Nitrogen ratio; Chlorophyll a, per dry mass; Chlorophyll c2, per dry mass; Chlorophyll Fluorescence System (I-PAM), Walz, Imaging-PAM fluorometer; CHSN Elemental Analyser, Elementar Analysen systeme GmbH, Germany, vario EL III; Date/time end, experiment; Date/time start, experiment; Enzymatic digestion; FACE-IT; Family; Fucoxanthin, per dry mass; Fv/Fm; High performance liquid chromatography (HPLC), Agilent Technologies, 1200 Series; coupled with Aminex Fast Carbohydrate analysis pillar HPAP (100 х 7,8 mm, 9 μ), BioRad front pillar: Phenomenex, Carbo-Pb2+ 4 x 3.00 mm i.d; High performance liquid chromatography (HPLC), VWR Hitachi International, LaChromElite® system, L-2200 autosampler [cooled], DA-detector L-2450, [Pillar: Spherisorb® ODS-2 (250 × 4.6 mm, 5 μm), Waters]; ImageJ (Version 1.52a); laminarin; Laminarin, per dry mass; Location; Mannitol; Mannitol, per dry mass; Maximum quantum yield of photosystem II; Nitrogen, total, per dry mass; Pigments; polar night; Porsangerfjorden_1; Porsangerfjorden_2; Porsangerfjorden_3; Porsangerfjorden, Norway; Precision Balance, Sartorius, MC 21S; Replicate; Species; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Treatment: light:dark cycle; Treatment: temperature; Type of study; Violaxanthin, per dry mass; Zeaxanthin, per dry mass |
| url | https://doi.org/10.1594/PANGAEA.972789 |