Guardado en:
| Autores principales: | , , , , |
|---|---|
| Formato: | Recurso digital |
| Lenguaje: | |
| Publicado: |
Zenodo
2026
|
| Acceso en línea: | https://doi.org/10.1002/adhm.202404870 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
Tabla de Contenidos:
- <p><span dir="ltr">Negative- and positive-staining transmission electron microscopy </span><span dir="ltr">(ns/psTEM) is a cornerstone of research and diagnostics, enabling </span><span dir="ltr">nanometer-resolution analysis of organic specimens from nanoparticles to </span><span dir="ltr">cells without requiring costly cryo-equipment. For nearly 70 years, uranyl salts </span><span dir="ltr">like uranyl acetate (UA) have been the gold-standard ns/psTEM-stains.</span><br><span dir="ltr">However, mounting safety concerns due to their high toxicity and radioactivity </span><span dir="ltr">have led to stricter regulations and expensive licensing requirements. </span><span dir="ltr">Consequently, there is an urgent global demand for safer, more sustainable </span><span dir="ltr">stains that deliver uranyl-comparable, high-quality ns/psTEM. Here, the </span><span dir="ltr">commercially available stain-alternatives UranyLess, UAR, UA-Zero, PTA, </span><span dir="ltr">STAIN 77, Nano-W, NanoVan, and lead citrate are systematically assessed </span><span dir="ltr">against UA. The stains are evaluated regarding their contrast, resolution, </span><span dir="ltr">stain-distribution, and ease-of-use in ns/psTEM across a diverse sample set, </span><span dir="ltr">including polymethylmethacrylate-nanoplastics, </span><span dir="ltr">phosphatidylcholine-liposomes, Influenza-A viruses, globular ferritin, fibrillar </span><span dir="ltr">pyruvate kinase amyloids, and human lung-carcinoma cell-sections. It is </span><span dir="ltr">shown that for this variety of samples, a ready-to-use uranyl-alternative is </span><span dir="ltr">commercially available with comparable or even superior </span><span dir="ltr">ns/psTEM-performance to UA using an efficient staining-protocol.</span><br><span dir="ltr">Furthermore, the</span> <span dir="ltr">GUIDE4U</span> <span dir="ltr">tool is developed for the fast identification of the </span><span dir="ltr">appropriate uranyl-replacements for each sample of interest, saving </span><span dir="ltr">ns/psTEM-users time and costs while ensuring excellent staining results for </span><span dir="ltr">ultrastructural analysis, thereby further catalyzing the use of safer stains.</span></p> <p> </p> <p><span dir="ltr">Acknowledgements:</span></p> <p><span dir="ltr"><span dir="ltr">Work in the </span><span dir="ltr">Nanomaterial in Health Laboratory profited from funding by Empa and </span><span dir="ltr">the MetrINo project (23.00360, 22HLT04, received funding from the Eu</span><span dir="ltr">ropean Partnership on Metrology, co-financed by the European Union’s </span><span dir="ltr">Horizon Europe Research and Innovation Programme and SERI (REF-</span><span dir="ltr">1131-52104)). Dr. Gea Cereghetti was supported by an EMBO Fellowship </span><span dir="ltr">(EMBO ALTF 349–2023), and a UKRI Engineering and Physical Sciences </span><span dir="ltr">Research Council Fellowship (grant EP/Z000033/1).</span></span></p>