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| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Recurso digital |
| Language: | English |
| Published: |
Zenodo
2019
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| Subjects: | |
| Online Access: | https://doi.org/10.1002/jcb.28273 |
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Table of Contents:
- We investigated the effects of photobiomodulation therapy (PBMT) and conditioned medium (CM) of human bone marrow mesenchymal stem cells (hBM-MSC) individually and/or in combinationon the stereological parametersand the expression of basic fibroblast growth factor (bFGF), hypoxia-inducible factor (HIF-1?), and stromal cellderived factor-1? (SDF-1?) in a wound model infected with methicillin-resistant Staphylococcus aureus (MRSA) in diabetic rats.CM was provided by culturing hBM-MSCs. Type 1 diabetes mellitus (T1DM) was induced in 72 rats, divided into four groups,harboring 18 rats each: group 1 served as a control group, group 2 received PBMT, group 3 received CM, and group 4 received CM + PBMT. On days 4, 7, and 15, six animals from each group were euthanized and the skin samples were separated for stereology examination and gene expression analysis by real-time polymerase chain reaction. In the CM + PBMT, CM, and PBMT groups, significant decreases were induced in the number of neutrophils (1460 93, 1854 138, 1719 248) and macrophages (539 69, 804 63, 912 41), and significant increases in the number of fibroblasts (1073 116, 836 75, 912 41) and angiogenesis (15 230 516, 13 318 1116, 14 041 867), compared with those of the control group (2690 371, 1139 145, 566 90, 12 585 1219). Interestingly, the findings of the stereological examination in the CM + PBMT group were statistically more significant than thosein the other groups. In the PBMT group, in most cases, the expression of bFGF, HIF-1?, and SDF-1?, on day 4 (27.7 0.14, 28.8 0.52, 27.5 0.54) and day 7 (26.8 1.4, 29.6 1.4, 28.3 1.2) were more significant than thosein the control (day 4, 19.3 0.42, 25.5 0.08, 22.6 0.04; day 7, 22.3 0.22, 28.3 0.59, 24.3 0.19) and other treatment groups. The application of PBMT + CM induced anti-inflammatory and angiogenic activities, and hastened wound healing process in a T1 DM model of MRSA infected wound. 2018 Wiley Periodicals, Inc.