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| Main Authors: | , , , , |
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| Format: | Recurso digital |
| Language: | |
| Published: |
Zenodo
1992
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| Online Access: | https://doi.org/10.5281/zenodo.14651276 |
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Table of Contents:
- <p>Routine use of a central venous catheter (CVC) is frequent in patients receiving an allogeneic bone marrow transplantation; cyclosporine (CyA) is used in the prevention of graft-versus-host disease in these patients. In this study we have compared the concentrations of CyA assayed from samples obtained simultaneously from CVC and from peripheral veins. This study conducted in 11 bone marrow-transplanted patients using a CVC made of silicone in 4 of them and CVCs made of polyurethane in the rest. The determination of CyA parent was performed by radioimmunoassay and that of CyA parent plus metabolites by fluorescence polarization immunoassay. CyA parent concentrations were significatively higher in samples from CVC than in samples from peripheral veins (470 and 184 ng/ml respectively). Similar findings were obtained when CyA parent plus metabolites were measured (829 and 506 ng/ml respectively). The correlation between all the samples from CVC and those from peripheral vein was r=0.19 for CyA parent, and r=0.30 for CyA parent plus metabolites, without significance (NS). Due to the great difference found between CyA concentrations in samples from CVC and peripheral veins, and to their poor correlation, it is not possible to apply a "conversion factor" to determine concentrations of peripheral veins through CVC concentrations. Routine monitoring of whole blood levels of samples obtained from peripheral vein is recommended to prevent to occurrence of immunosuppressive inefficacy.</p>