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Detaylı Bibliyografya
Yazar: Ghaly, Timothy
Materyal Türü: Recurso digital
Dil:
Baskı/Yayın Bilgisi: Zenodo 2025
Online Erişim:https://doi.org/10.5281/zenodo.15054613
Etiketler: Etiketle
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İçindekiler:
  • <p>Metagenome-assembled genome (MAG) sequences from the global soil microbiome were obtained from the <a href="https://doi.org/10.1038/s41467-023-43000-z" target="_blank" rel="noopener">Soil Microbial Dark Matter MAG</a> and <a href="https://doi.org/10.1101/2024.12.19.629313" target="_blank" rel="noopener">Microflora Danica long-read MAG</a> catalogues, and filtered to retain only high-quality (HQ) MAGs based on <a href="https://doi.org/10.1038/nbt.3893" target="_blank" rel="noopener">MIMAG</a> standards. That is, genomes that had >90% CheckM2 completeness and <5% CheckM2 contamination, and had the 23S, 16S, and 5S rRNA genes, and at least 18 different tRNA genes. MAGs were dereplicated at approximately species-level, using clustering thresholds of 95% average nucleotide identity (ANI) and 50% aligned fraction (AF).<br><br>MAG sequences are within the compressed directory, 'MAGs.tar.gz'.<br>Pooled predicted protein sequences are in 'HQ_MAGs.prots.faa.gz'.</p>