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| Main Authors: | , |
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| Formato: | Recurso digital |
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Zenodo
2020
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| Subjects: | |
| Acceso en liña: | https://doi.org/10.5281/zenodo.15136013 |
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Table of Contents:
- <p><b>Table 2.</b> Sequences of the primers used for PCR amplification and sequencing, as well as PCR conditions.</p><table><thead><tr><th colspan="1" rowspan="2">DNA region</th><th colspan="1" rowspan="2">Primer name</th><th colspan="1" rowspan="2">Primer Sequence 5 ’-3’</th><th colspan="1" rowspan="2">Reference</th><th colspan="6" rowspan="1">PCR Conditions</th></tr><tr><th colspan="1" rowspan="1">Pre-melting</th><th colspan="1" rowspan="1">Denaturation (I)</th><th colspan="1" rowspan="1">Primer Annealing (II)</th><th colspan="1" rowspan="1">Primer Extension (III)</th><th colspan="1" rowspan="1">Cycles (I + II + III)</th><th colspan="1" rowspan="1">Final Extension</th></tr></thead><tbody><tr><td colspan="1" rowspan="2">ETS</td><td colspan="1" rowspan="1">18S-IGS</td><td colspan="1" rowspan="1">GAGACAAGCATATGACTACTGGCAGGATCAACCAG</td><td colspan="1" rowspan="1">Baldwin and Markos (1998)</td><td colspan="1" rowspan="2">94 °C (3 min)</td><td colspan="1" rowspan="2">94 °C (1 min)</td><td colspan="1" rowspan="2">55 °C (1 min)</td><td colspan="1" rowspan="2">72 °C (1.5 min)</td><td colspan="1" rowspan="2">30</td><td colspan="1" rowspan="2">72 °C (7 min)</td></tr><tr><td colspan="1" rowspan="1">ETS-Dio</td><td colspan="1" rowspan="1">GCTTGTGCATCGAACGGTTGG</td><td colspan="1" rowspan="1">Queiroz et al. (2015)</td></tr><tr><td colspan="1" rowspan="6">ITS</td><td colspan="1" rowspan="1">17SE (F)</td><td colspan="1" rowspan="1">ACGAATTCATGGTCCGGTGAAGTGTTCG</td><td colspan="1" rowspan="1">Sun et al. (1994)</td><td colspan="1" rowspan="4">94 °C (3 min)</td><td colspan="1" rowspan="4">94 °C (1 min)</td><td colspan="1" rowspan="4">52 °C (40 s)</td><td colspan="1" rowspan="4">72 °C (2.5 min)</td><td colspan="1" rowspan="4">28</td><td colspan="1" rowspan="4">72 °C (7 min)</td></tr><tr><td colspan="1" rowspan="1">26SE (R)</td><td colspan="1" rowspan="1">TAGAATTCCCCGGTTCGCTCGCCGTTAC</td><td colspan="1" rowspan="1">Sun et al. (1994)</td></tr><tr><td colspan="1" rowspan="1">5.8S</td><td colspan="1" rowspan="1">ACGACTCTCGGCAAC</td><td colspan="1" rowspan="1">Sun et al. (1994)</td></tr><tr><td colspan="1" rowspan="1">5.8R</td><td colspan="1" rowspan="1">GCGTGACGCCCAGGC</td><td colspan="1" rowspan="1">Sun et al. (1994)</td></tr><tr><td colspan="1" rowspan="1">SSF</td><td colspan="1" rowspan="1">GTCGTAACAAGGTTTCCGTAG</td><td colspan="1" rowspan="1">Kollipara et al. (1997)</td><td colspan="6" rowspan="2">Following manufacturer’s protocol for sequencing</td></tr><tr><td colspan="1" rowspan="1">LSR</td><td colspan="1" rowspan="1">GTTAGTTTCTTTTCCTCC</td><td colspan="1" rowspan="1">Kollipara et al. (1997)</td></tr><tr><td colspan="1" rowspan="6"><i>trnK/ matK</i></td><td colspan="1" rowspan="1">matK685F</td><td colspan="1" rowspan="1">GTATCGCACTATGTATTATTTGA</td><td colspan="1" rowspan="1">Wojciechowski et al. (2004)</td><td colspan="1" rowspan="6">94 °C (3 min)</td><td colspan="1" rowspan="6">94 °C (40 s)</td><td colspan="1" rowspan="6">55 °C (45 s)</td><td colspan="1" rowspan="6">72 °C (1 min)</td><td colspan="1" rowspan="6">36</td><td colspan="1" rowspan="6">72 °C (7 min)</td></tr><tr><td colspan="1" rowspan="1">matK4La</td><td colspan="1" rowspan="1">CCTTCGATACTGGGTGAAAGAT</td><td colspan="1" rowspan="1">Wojciechowski et al. (2004)</td></tr><tr><td colspan="1" rowspan="1">matK1100L</td><td colspan="1" rowspan="1">TTCAGTGGTACGGAGTCAAATG</td><td colspan="1" rowspan="1">Wojciechowski et al. (2004)</td></tr><tr><td colspan="1" rowspan="1">matK4R</td><td colspan="1" rowspan="1">CATCTTTCACCCAGTAGCGAAG</td><td colspan="1" rowspan="1">Hu et al. (2000)</td></tr><tr><td colspan="1" rowspan="1">matK1932R</td><td colspan="1" rowspan="1">CAGACCGGCTTACTAATGGG</td><td colspan="1" rowspan="1">Hu et al. (2000)</td></tr><tr><td colspan="1" rowspan="1">trnK2R</td><td colspan="1" rowspan="1">CCCGGAACTAGTCGGATG</td><td colspan="1" rowspan="1">Wojciechowski et al. (2004)</td></tr></tbody></table>