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| Формат: | Recurso digital |
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| Опубліковано: |
Zenodo
2025
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| Предмети: | |
| Онлайн доступ: | https://doi.org/10.5281/zenodo.15428356 |
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Зміст:
- <p>The RNA World hypothesis posits that RNA-based systems, such as ribosomes, could sustain early life without additional molecular support. We test this by simulating a protocell composed solely of a modern ribosome (large and small subunits, ~4,500 nt rRNA, excluding mRNA, tRNA, proteins, or other elements) in hydrothermal vents (80 ± 15°C, pH 6.0 ± 0.5, oxidative stress 0.45 ± 0.18 mM), ice (-5 ± 3°C, pH 7.0 ± 0.4, oxidative stress 0.08 ± 0.04 mM), and wet-dry cycles (25 ± 10°C, pH 6.8 ± 0.6, oxidative stress 0.35 ± 0.15 mM). An Agent-Based Model (ABM) simulates 1,000 protocells over 100,000 steps (~100 division cycles) with 1,500 replicates. Without supporting elements, ribosomes exhibit negligible function: median survival of 20,000–40,000 steps, proto-translation rates of 0.001–0.003 mM/hr, rRNA mutation rates of 5.5–6.0 × 10⁻⁷, and lineage divergence of 20–30%. Statistical analyses (ANOVA, regression, Kaplan-Meier, bootstrap) confirm robust failure (p < 0.001, power >0.85, 95% CI ±1.0–2.0%), with ice marginally better but insufficient. Compared to supported systems (970,000 steps, 0.068 mM/hr), ribosome-only protocells are 96–98% less viable. These simulations statistically refute the RNA World hypothesis, supporting the Matter World Hypothesis (MWH) that molecular synergy is essential. Results are computational and require experimental validation.</p>