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Zenodo
2025
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| Online Erişim: | https://doi.org/10.5281/zenodo.15865482 |
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- <p><i>Tuber conditum</i> T. J. Yuan, S. H. Li & X. H. Wang sp. nov.</p><p>Fig. 4 a – f</p><p><b>Typification.</b></p><p>China • Yunnan Province: Changning County (38.3903°N, 102.3535°E), in soil under mixed forest, with <i>Pinus yunnanensis</i> dominant, 20 Sep 2020, S. H. Li L 3385 (holotype YAAS L 3385, paratype YAAS L 3682).</p><p><b>Gene sequences ex holotype.</b></p><p>ON 454665 (ITS); ON 428901 (LSU); ex paratype: ON 454667 (ITS).</p><p><b>Etymology.</b></p><p>Conditum, Latin, refers to the acrid smell of ascocarp.</p><p><b>Diagnosis.</b></p><p><i>Tuber conditum</i> differs from other species by having no cystidia on surface of ascomata, thinner peridium, and larger ellipsoid ascospores.</p><p><b>Description.</b></p><p><i>Ascomata</i> (Fig. 4 a) 3–4 cm diam., subglobose, hypogeous, grey-white to grey-brown (4 A 2-4; 4 B 3-4), surface smooth, with a few pinholes; acrid smell, taste not recorded. <i>Peridium</i> (Fig. 4 b) one-layered, 120–200 µm thick, prosenchymatous, composed of interwoven hyphae 1.5–2.5 µm broad with thin-walled, and lumens 1.5–2.5 (3.5) µm diam, with ellipsoid or irregular cells of 15 × 10 μm diam. Sometimes intermixed, wall thickness 1–2 μm. <i>Gleba</i> (Fig. 4 a, c) solid, firm, brown to black (6 E 7-8; (5-9) F 8) at maturity, marbled with whitish narrow veins. <i>Asci</i> (Fig. 4 d – e) subglobose or irregular, 1–4 spored, hyaline, thin-walled or occasionally with walls as thick as 2 μm, sessile stalk, 55–100 × 45–75 μm (n = 30). <i>Ascospores</i> (Fig. 4 d – f) subglobose or ellipsoid, pale yellow (2 A 2-4), yellow-brown (5 D 6-8; 5 E 7-8) at maturity, excluding ornamentation, 49–66.5 × 34.5–60 μm, <i>Q</i> = 1.02–1.58 (n = 54), in one-spored asci, 33–54.5 × 30–47 μm, <i>Q</i> = 1.06–1.35 (n = 50), in two-spored asci, 21.5–44 × 21–37 μm, <i>Q</i> = 1.00–1.26 (n = 60), in three-spored asci, 20.5–40.0 × 20–35.5 μm, <i>Q</i> = 1.01–1.30 (n = 40), in four-spored asci, Q = 1.15 ± 0.23, reticulate ornamentation 2–5 μm in height, composed of irregular hexagonal meshes, 6–8 along the spore length and 3–6 along the breadth.</p><p><b>Distribution and habitat.</b></p><p>China: Yunnan province, Changning county, hypogeous, in the soil in woods dominated by <i>Pinus yunnanensis</i>.</p><p><b>Notes.</b></p><p><i>Tuber conditum</i> is phylogenetically closely related to <i>Tuber tomentosum</i> (Kinoshita et al. 2011), <i>T. qujingense</i>, and <i>T. songlu</i> (Wan et al. 2021). However, <i>T. conditum</i> has no cystidia on the surface of ascomata and larger ellipsoid ascospores, differing from <i>T. tomentosum</i> (Kinoshita et al. 2011; Sasaki et al. 2016). <i>Tuber conditum</i> differs from <i>T. qujingense</i> and <i>T. songlu</i> by its thinner peridium (120–200 µm) and larger ascospores (49–66.5 × 34.5–60 μm in one-spored asci). <i>Tuber qujingense</i> has a greyish-white ascomata, brown snowflake-shaped gleba, and prosenchymatous peridium, fusiform, and <i>T. songlu</i> has dense spine-like dermatocystidia, which are completely different from each other. In addition, DNA sequence analysis revealed the similarity between two species is less than 82.73 % (<i>T. qujingense</i>) and 93 % (<i>T. songlu</i>) in ITS sequences, strongly supporting the separation of <i>T. conditum</i> from the Chinese species of <i>T. qujingense</i> and <i>T. songlu.</i> Phylogenetic analysis (Figs 1, 2) also suggested that <i>T. conditum</i> is a distinct species because all sequences of this new species clustered within a strong bootstrap (BS ≥ 99 % and PP = 1.0). The truffle has the acrid smell, which might be a good identifying feature for the fungus.</p>