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| Formato: | Recurso digital |
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| Publicado: |
Zenodo
2025
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| Acceso en liña: | https://doi.org/10.5281/zenodo.17409015 |
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Table of Contents:
- <div> <p><em><span>Size Exclusion Chromatography (SEC), also known as gel filtration or gel permeation chromatography, is a powerful analytical technique that separates molecules according to their hydrodynamic size without chemical or ionic interactions. This manuscript provides a concise review of SEC principles, instrumentation, and recent advancements, emphasizing its role in the characterization of proteins, polymers, nanoparticles, and biotherapeutics. SEC’s non-destructive mechanism preserves sample integrity, making it essential for macromolecular analysis. Developments in multi-angle light scattering (MALS), ultra-high performance SEC (UHP-SEC), and bio-inert column technology have further improved precision and resolution. The review also highlights practical aspects such as column selection, mobile phase optimization, and detector configurations. Despite limitations in resolving similarly sized species, SEC remains indispensable in pharmaceutical and polymer research.</span></em></p> <p><em><span>Keywords: Size Exclusion Chromatography, Gel Filtration, SEC-MALS, Polymer Analysis, Protein Purification, Biopharmaceuticals.</span></em></p> </div>