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| 主要な著者: | , , , , |
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| フォーマット: | Recurso digital |
| 言語: | 英語 |
| 出版事項: |
Zenodo
2017
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| 主題: | |
| オンライン･アクセス: | https://doi.org/10.5281/zenodo.18857739 |
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- <p><span lang="EN-US">Malaria is an important health and development challenge in Africa. Animal models, most particularly mice, have long been employed to study malaria pathogenesis. This paper describes clinical manifestations due to <em>Plasmodium berghei </em>ANKA infection in juvenile mice as a model for understanding the complications of congenital malaria in neonates. <span>Forty-five juvenile mice (5-7 days old) were acquired from University College Hospital, Ibadan and </span>injected with 2×10<sup>7 </sup>(0.2 ml) <em>Plasmodium berghei</em> ANKA parasitized red blood cells (PRBCs). The mice were then transported to the study site, kept in well-ventilated cages and fed daily with a balanced ration<span>. Post<em>-P. berghei</em><span> </span>infection, the mice were monitored daily for mortality<em>.</em><span> </span>Clinical manifestations of experimental cerebral malaria (ECM) were assessed and confirmed if at least ruffled fur, hunching, wobbly gait, limb paralysis, convulsions, or coma was observed. Each sign was given a score of 1. Animals with scores ≥4 were considered to have severe ECM. In the experiment, </span><span>20 (44%) mice were lost due to natural cause (i.e. stress) at day 2. </span>Between day 4 and 9, 25 (56%) of the study mice presented clinical signs of ECM. This included: <span>ruffled fur – 25 (100%), hunching - 21 (84%), wobbly gait - 17 (68%), limb paralysis - 20 (80%), convulsions - 25 (100%)</span>. Survival rate and severity of ECM in the mice differs, 22 (88.0%) had s<span>evere ECM and 3 (12.0%) had </span>mild ECM. This study has shown that parasite establishment and malaria complications can manifest as early as 4 days’ <span>post<em> P. berghei</em> infection in </span><span>5-7 days old</span><span> mice. </span></span></p>