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| Main Author: | |
|---|---|
| Format: | Recurso digital |
| Language: | English |
| Published: |
Zenodo
2026
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| Online Access: | https://doi.org/10.5281/zenodo.18874537 |
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Table of Contents:
- <p dir="auto">The Synaptosome Imaging Analysis App (vSynApp) is designed to facilitate the analysis of synatposomal proteins imaged using immunofluorescence.</p> <p dir="auto">This app provides a suite of tools to analyze immunofluorescence microscopy images of synaptosomes. The primary goal is to analyse fluorescence on a per-structure basis, enabling researchers to assess signal thresholds, potential antibodies interactions, co-localizations, and the presence of bleedthrough between channels. Bleedthrough refers to the phenomenon where the signal from one fluorescence channel is detected in another channel, which can lead to false-positive interpretations of co-localization.</p> <p dir="auto">The app incorporates a variety of statistical and computational methods to analyze the data. These methods help distinguish between specific staining and background noise or non-specific staining, ensuring accurate interpretation of the microscopy data. </p> <p dir="auto">The app is currently tailored for data analysed using IJ-Toolset_SynaptosomesMacro.</p>