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Autore principale: PARASURAM RAJAM RADHIKA, UMAMAHESWARI THANGAM, BHARATH MADESHWARAN, T. KEERTHIVASAN
Natura: Recurso digital
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Pubblicazione: Zenodo 2026
Accesso online:https://doi.org/10.5281/zenodo.19081708
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author PARASURAM RAJAM RADHIKA, UMAMAHESWARI THANGAM, BHARATH MADESHWARAN, T. KEERTHIVASAN
author_facet PARASURAM RAJAM RADHIKA, UMAMAHESWARI THANGAM, BHARATH MADESHWARAN, T. KEERTHIVASAN
contents <p>The present research communicates the formation of Kojic acid-loaded lipid-polymer hybrid nanoparticles <br>(LPHNPs) as an innovative system for the halt of human skin cancer. The production of LPHNPs was performed by <br>applying the combination of hot homogenization and ultrasonication techniques with Compritol 888 ATO, <br>Phospholipon 90G, Tween 80, and ethyl acetate. The hybrid approach combined the advantages of the high drug<br>loading capacity of polymers and the safety and stability of lipids. The transformation of the drug and excipient was <br>confirmed by the FTIR analysis. DLS indicated the presence of particles with sizes ranging from 120 to 210 nm and <br>a low PDI of less than 0.3, which was a sign of uniformity. The zeta potential varying from -12.1 to -18.4 mV <br>pointed to moderate colloidal stability while SEM images displayed smooth and spherical nanoparticles. The XRD <br>patterns indicated the partial amorphization of Kojic acid, which resulted in improved solubility and dissolution <br>potential. The in vitro cytotoxicity with the MTT assay on the A375 human melanoma cells indicated a dose<br>dependent effect, with the LPHNPs being much more potent in inhibiting cell growth than free Kojic acid. The <br>formulation F3 produced the strongest cytotoxic effect on the cells, similar to the effect produced by cisplatin, and <br>thus the formulation was selected as optimized. In conclusion, the Kojic acid-loaded LPHNPs, particularly F3, <br>inherited the traits of excellent stability, enhanced solubility, and pronounced anticancer activity, thus positioning <br>LPHNPs as a viable option for safe and effective delivery in skin cancer therapy. </p>
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spellingShingle INHIBITION OF SKIN CANCER CELL PROLIFERATION BY KOJIC ACID-ENCAPSULATED LIPID-POLYMER HYBRID NANOPARTICLES
PARASURAM RAJAM RADHIKA, UMAMAHESWARI THANGAM, BHARATH MADESHWARAN, T. KEERTHIVASAN
<p>The present research communicates the formation of Kojic acid-loaded lipid-polymer hybrid nanoparticles <br>(LPHNPs) as an innovative system for the halt of human skin cancer. The production of LPHNPs was performed by <br>applying the combination of hot homogenization and ultrasonication techniques with Compritol 888 ATO, <br>Phospholipon 90G, Tween 80, and ethyl acetate. The hybrid approach combined the advantages of the high drug<br>loading capacity of polymers and the safety and stability of lipids. The transformation of the drug and excipient was <br>confirmed by the FTIR analysis. DLS indicated the presence of particles with sizes ranging from 120 to 210 nm and <br>a low PDI of less than 0.3, which was a sign of uniformity. The zeta potential varying from -12.1 to -18.4 mV <br>pointed to moderate colloidal stability while SEM images displayed smooth and spherical nanoparticles. The XRD <br>patterns indicated the partial amorphization of Kojic acid, which resulted in improved solubility and dissolution <br>potential. The in vitro cytotoxicity with the MTT assay on the A375 human melanoma cells indicated a dose<br>dependent effect, with the LPHNPs being much more potent in inhibiting cell growth than free Kojic acid. The <br>formulation F3 produced the strongest cytotoxic effect on the cells, similar to the effect produced by cisplatin, and <br>thus the formulation was selected as optimized. In conclusion, the Kojic acid-loaded LPHNPs, particularly F3, <br>inherited the traits of excellent stability, enhanced solubility, and pronounced anticancer activity, thus positioning <br>LPHNPs as a viable option for safe and effective delivery in skin cancer therapy. </p>
title INHIBITION OF SKIN CANCER CELL PROLIFERATION BY KOJIC ACID-ENCAPSULATED LIPID-POLYMER HYBRID NANOPARTICLES
url https://doi.org/10.5281/zenodo.19081708