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| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Recurso digital |
| Language: | |
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Zenodo
2026
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| Online Access: | https://doi.org/10.5281/zenodo.19600813 |
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Table of Contents:
- <p>This Zenodo deposit contains a publicly available description of the Dataset:</p> <p><strong>Title:</strong> "iSCORE-PD: NGS genotyping and zygosity testing of isogenic hESC clones".</p> <p><strong>Description:</strong> Targeted DNA sequencing data for the iSCORE-PD isogenic hESC clone collection, encompassing three complementary analyses: (1) NGS genotyping of 53 mutant clones across 11 PD-linked genes via amplicon sequencing on Illumina iSeq 100 with CRISPResso2 analysis, (2) zygosity testing of a subset of clones in 6 genes via SNP-PCR (NGS or Sanger) to assess zygosity near CRISPR edit sites, and (3) Sanger sequencing of the wild-type allele in 5 DJ1/PARK7 heterozygous clones.</p> <blockquote> <p>This dataset is made available to researchers via the ASAP CRN Cloud: <a href="https://cloud.parkinsonsroadmap.org">cloud.parkinsonsroadmap.org</a>. Instructions for how to request access can be found in the <a href="https://storage.googleapis.com/asap-public-assets/wayfinding/ASAP-CRN-Cloud-User-Manual.pdf">User Manual</a>.</p> <p>This research was funded by the Aligning Science Across Parkinson's Collaborative Research Network (ASAP CRN), through the Michael J. Fox Foundation for Parkinson's Research (MJFF).</p> <p>This Zenodo deposit was created by the ASAP CRN Cloud staff on behalf of the dataset authors. It provides a citable reference for a CRN Cloud Dataset</p> </blockquote>