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| Үндсэн зохиолч: | |
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| Формат: | Recurso digital |
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Zenodo
2026
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| Нөхцлүүд: | |
| Онлайн хандалт: | https://doi.org/10.5281/zenodo.20043467 |
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Шошго нэмэх
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| _version_ | 1866901376809828352 |
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| author | Gajanan Shelke, Gopal Dhandar, Gaurav Chaukhande*, Dipak Lokhande, Dhananjay Mhaske, Prashant Waghmode, R. H. Kale |
| author_facet | Gajanan Shelke, Gopal Dhandar, Gaurav Chaukhande*, Dipak Lokhande, Dhananjay Mhaske, Prashant Waghmode, R. H. Kale |
| contents | <p class="MsoNormal"><span>Antimicrobial resistance (AMR) is a growing global health threat that demands exploration of novel and plant-based antimicrobial agents. The present study evaluated and compared the antimicrobial activity of aqueous and ethanolic extracts of four medicinal plants — Ocimum sanctum (Tulsi), Curcuma longa (Turmeric), Azadirachta indica (Neem), and Allium sativum (Garlic) — against six clinically important pathogenic microorganisms: Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Klebsiella pneumoniae (ATCC 13883), Candida albicans (ATCC 10231), and Aspergillus niger (ATCC 6275). Standard agar disc diffusion (Kirby–Bauer method) was used to measure zones of inhibition (ZOI), while minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC/MFC) were determined by broth microdilution in 96-well plates. Among the extracts, Allium sativum ethanolic extract exhibited the highest broad-spectrum activity (ZOI: 22.6 ± 1.2 mm against S. aureus) with lowest MIC (0.78 mg/mL). Curcuma longa showed significant antifungal activity. All extracts demonstrated promising antimicrobial potential compared to standard antibiotics. The results validate the traditional use of these plants and support their application in developing plant-based antimicrobial formulations.</span></p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_20043467 |
| institution | Zenodo |
| language | |
| publishDate | 2026 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | Comparative Evaluation of Antimicrobial Activity of Selected Medicinal Plant Extracts Against Pathogenic Microorganisms Using Standard Microbiological Methods Gajanan Shelke, Gopal Dhandar, Gaurav Chaukhande*, Dipak Lokhande, Dhananjay Mhaske, Prashant Waghmode, R. H. Kale Antimicrobial Activity, Disc Diffusion, MIC, Medicinal Plant Extracts, Kirby-Bauer, Phytochemicals, Antimicrobial Resistance <p class="MsoNormal"><span>Antimicrobial resistance (AMR) is a growing global health threat that demands exploration of novel and plant-based antimicrobial agents. The present study evaluated and compared the antimicrobial activity of aqueous and ethanolic extracts of four medicinal plants — Ocimum sanctum (Tulsi), Curcuma longa (Turmeric), Azadirachta indica (Neem), and Allium sativum (Garlic) — against six clinically important pathogenic microorganisms: Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Klebsiella pneumoniae (ATCC 13883), Candida albicans (ATCC 10231), and Aspergillus niger (ATCC 6275). Standard agar disc diffusion (Kirby–Bauer method) was used to measure zones of inhibition (ZOI), while minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC/MFC) were determined by broth microdilution in 96-well plates. Among the extracts, Allium sativum ethanolic extract exhibited the highest broad-spectrum activity (ZOI: 22.6 ± 1.2 mm against S. aureus) with lowest MIC (0.78 mg/mL). Curcuma longa showed significant antifungal activity. All extracts demonstrated promising antimicrobial potential compared to standard antibiotics. The results validate the traditional use of these plants and support their application in developing plant-based antimicrobial formulations.</span></p> |
| title | Comparative Evaluation of Antimicrobial Activity of Selected Medicinal Plant Extracts Against Pathogenic Microorganisms Using Standard Microbiological Methods |
| topic | Antimicrobial Activity, Disc Diffusion, MIC, Medicinal Plant Extracts, Kirby-Bauer, Phytochemicals, Antimicrobial Resistance |
| url | https://doi.org/10.5281/zenodo.20043467 |