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Zenodo
2020
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| Acesso em linha: | https://doi.org/10.5281/zenodo.3809595 |
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Sumário:
- <p><b>DESCRIPTION OF <i>NONOMURAEA DESERTI</i> SP. NOV.</b></p><p><i>Nonomuraea deserti</i> sp. nov. (de.ser <i>′</i> ti. L. gen. n. <i>deserti</i> of a desert, referring to the source of the type strain).</p><p>Aerobic, Gram-stain-positive, non-motile actinobacterium that forms extensively branched substrate and aerial mycelia. Aerial hyphae differentiated to hooked chains of spores with a smooth surfaces. Good growth occurs on ISP 2, 3, modified Bennett’s and nutrient agar and moderate growth on ISP</p><p>4–7, Czapek’s and tryptic soy agar, substrate mycelia may be brown, cream or yellow. Does not produce diffusible or melanoid pigments. Grows from 28–37 °C (optimum, 28 °C), from pH 6.0–8.0 (optimum, pH 7.0) and in the presence of up to 3 % (w/v) NaCl. Hydrolyses aesculin and arbutin, reduces nitrate but does not form H 2 S and does not hydrolyse allantoin or urea. Gelatin, hypoxanthine and xylan are degraded, but not adenine, casein, chitin, guanine, starch, Tweens 40 or 80 or xanthine. D-Arabinose, cellobiose, dextran, D-fructose, D-glucose, lactose, D-mannitol and raffinose are utilized as sole carbon and energy sources, but not adonitol, L-arabinose, dextrin, D-galactose, L-glutamine, maltose, myo-inositol, D-mannose, melezitose, melibiose, inulin, L-rhamnose, D-sorbitol, L-sorbose, sodium succinate, sucrose, xylitol or xylose. L-Arginine, L-asparagine, L-cysteine, glycine, L-hydroxyproline, L-methionine, L-phenylalanine, L-proline,</p><p>*Data taken from Le Roes and Meyers [77].</p><p>†Data taken from Qin <i>et al</i>. [18].</p><p>‡Data taken from Li <i>et al</i>. [29].</p><p>L-serine, L-tyrosine and L-valine are utilized as sole nitrogen sources, but not L-alanine, L-histidine or α-iso-leucine. The predominant menaquinones are MK-9(H 4) and MK-9(H 6). The polar lipid profile includes diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, dihydroxy-phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, three unidentified glycophospholipids, an unidentified phospholipid, an unidentified glycolipid and four unidentified lipids. Whole-cell hydrolysates contain <i>meso</i> -A 2 pm, glucose, mannose, madurose, ribose and galactose. The major fatty acids are iso-C 16:0, iso <i>-</i> C 16:0 2OH and C 17:0 10-methyl. The DNA G+C content of strain KC310 T is 70.9 mol% and the genome size 9.69 Mbp.</p><p>The type strain, KC310 T (= CGMCC 4.7331 T = DSM 102919 T =KCTC 39774 T), was isolated from desert soil sample collected from the Karakum Desert, Turkmenistan. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain KC310 T is MG770639 and draħ genome sequence accession number SMKO00000000.</p>