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Zenodo
2023
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| Acesso em linha: | https://doi.org/10.5281/zenodo.8403865 |
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Sumário:
- <p><b><i>Epicoccum nigrum</i> Link, Mag. Ges. Naturf. Freunde Berlin 7: 32 (1816) (Figure 8)</b></p><p>Index Fungorum number: IF226758</p><p><i>Endophytic</i> on fresh leaves of <i>Coriaria nepalensis</i> <b>Sexual</b> <b>morph</b>: Unknown. <b>Asexual morph on PDA</b>: <i>Mycelium</i> composed of 2–7 µm (<i>x</i> = 5 µm; n = 15) wide, branched, hyaline, smooth and thick-walled hyphae, constricted at the septum. <i>Conidiophores</i> micronematous, inconspicuous. <i>Conidiogenous cells</i> 3–6.5 × 3–6 μm (<i>x</i> = 5.5 × 4.5 µm; n = 20), phialidic, hyaline, rounded ends, thin-walled. <i>Conidia</i> 16–21 × 15–20 μm (<i>x</i> = 18.5 × 18 µm; n = 20), brown to dark brown, mostly ellipsoid, rarely globose to subglobose, septate, apparently constricted at the septum, verrucose and sometimes with a basal cell.</p><p><b>Culture characteristics:</b> Colonies on PDA, reaching around 20 mm diameter after 2 weeks at 20–25 ℃. Obverse: cream and yellowish mycelia, raised, lowly dense, effuse, irregular, fluffy. Reverse: sunk, dark brown at the centre, light brown at the margin. With golden brown pigments produced in PDA.</p><p><b>Distribution:</b> Russia (Hyde <i>et al.</i> 2018); China (This study).</p><p><b>Substratum:</b> <i>Acer negundo</i> (Sapindaceae) (Hyde <i>et al.</i> 2018); <i>Coriaria nepalensis</i> (This study).</p><p><b>Material examined:</b> China, Yunnan Province, Kunming, Kunyang, an abandoned and Open-Pit Phosphate Mine, on fresh leaves of <i>Coriaria nepalensis,</i> 22August 2022, C. J. Dao, 2 MS1 L2 (HKAS 129062), living culture KUNCC23- 13352. GenBank number: ITS: OR094462, LSU: OR094457, <i>rpb2</i>: OR095636, <i>tub2</i>: OR095641.</p><p><b>Notes:</b> Our isolate shares similar conidia with the <i>Epicoccum nigrum</i> forming brown to dark brown, ellipsoid, globose to subglobose, septate, verrucose conidia (Hyde <i>et al.</i> 2018). Based on the BLASTn search results of multi genes (LSU, ITS, <i>rpb2</i> and <i>tub2</i>), our isolate (KUNCC 23-13352) show 99–100% similarity to <i>E. nigrum</i> strains (CBS 231.59, CBS 125.82, CPO 10.815, LC 5180, MFLUCC 17-1171). In addition, the multi gene phylogenetic trees (ML and BI) indicate our isolate clusters with the <i>E. nigrum</i> strains (Figure 7). Therefore, we introduce our isolate as a new host record of <i>E. nigrum</i> based on BLASTn search results, morphological study and phylogenetic analyses.</p>