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| Main Authors: | , , , |
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| Format: | Preprint |
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2017
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| Subjects: | |
| Online Access: | https://arxiv.org/abs/1708.04693 |
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| _version_ | 1866913608289484800 |
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| author | Chowdhury, Shwetadwip Eldridge, Will J. Wax, Adam Izatt, Joseph A. |
| author_facet | Chowdhury, Shwetadwip Eldridge, Will J. Wax, Adam Izatt, Joseph A. |
| contents | Though structured illumination (SI) microscopy is a popular imaging technique conventionally associated with fluorescent super-resolution, recent works have suggested its applicability towards sub-diffraction coherent imaging with quantitative endogenous biological contrast. Here, we demonstrate that SI can efficiently integrate the principles of fluorescent super-resolution and coherent synthetic aperture to achieve 3D dual-modality sub-diffraction resolution, fluorescence and refractive-index (RI) visualizations of biological samples. Such a demonstration can enable future biological studies to synergistically explore molecular and biophysical/biochemical mechanisms at sub-diffraction resolutions. We experimentally demonstrate this framework by introducing a SI microscope capable of 3D sub-diffraction fluorescence and RI imaging, and verify its biological visualization capabilities by experimentally reconstructing 3D RI/fluorescence visualizations of fluorescent calibration microspheres as well as alveolar basal epithelial adenocarcinoma (A549) and human colorectal adenocarcinmoa (HT-29) cells, fluorescently stained for F-actin. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_1708_04693 |
| institution | arXiv |
| publishDate | 2017 |
| record_format | arxiv |
| spellingShingle | Structured illumination microscopy for dual-modality 3D sub-diffraction resolution fluorescence and refractive-index reconstruction Chowdhury, Shwetadwip Eldridge, Will J. Wax, Adam Izatt, Joseph A. Quantitative Methods Biological Physics Though structured illumination (SI) microscopy is a popular imaging technique conventionally associated with fluorescent super-resolution, recent works have suggested its applicability towards sub-diffraction coherent imaging with quantitative endogenous biological contrast. Here, we demonstrate that SI can efficiently integrate the principles of fluorescent super-resolution and coherent synthetic aperture to achieve 3D dual-modality sub-diffraction resolution, fluorescence and refractive-index (RI) visualizations of biological samples. Such a demonstration can enable future biological studies to synergistically explore molecular and biophysical/biochemical mechanisms at sub-diffraction resolutions. We experimentally demonstrate this framework by introducing a SI microscope capable of 3D sub-diffraction fluorescence and RI imaging, and verify its biological visualization capabilities by experimentally reconstructing 3D RI/fluorescence visualizations of fluorescent calibration microspheres as well as alveolar basal epithelial adenocarcinoma (A549) and human colorectal adenocarcinmoa (HT-29) cells, fluorescently stained for F-actin. |
| title | Structured illumination microscopy for dual-modality 3D sub-diffraction resolution fluorescence and refractive-index reconstruction |
| topic | Quantitative Methods Biological Physics |
| url | https://arxiv.org/abs/1708.04693 |