xSCYTE: Express Single-frame Cytometer through Tomographic Phase
Fuente:
arXiv
Salvato in:
| Autori principali: | , , , , , , , , , , , , , , , |
|---|---|
| Natura: | Preprint |
| Pubblicazione: |
2022
|
| Soggetti: | |
| Accesso online: | |
| Tags: |
Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne!!
|
| _version_ | 1866916467564347392 |
|---|---|
| author | Ge, Baoliang He, Yanping Deng, Mo Rahman, Md Habibur Wang, Yijin Wu, Ziling Yang, Yongliang Kuang, Cuifang Wong, Chung Hong N. Chan, Michael K. Ho, Yi-Ping Duan, Liting Yaqoob, Zahid So, Peter T. C. Barbastathis, George Zhou, Renjie |
| author_facet | Ge, Baoliang He, Yanping Deng, Mo Rahman, Md Habibur Wang, Yijin Wu, Ziling Yang, Yongliang Kuang, Cuifang Wong, Chung Hong N. Chan, Michael K. Ho, Yi-Ping Duan, Liting Yaqoob, Zahid So, Peter T. C. Barbastathis, George Zhou, Renjie |
| contents | Rapid, comprehensive, and accurate cell phenotyping without compromising viability, is crucial to many important biomedical applications, including stem-cell therapy, drug screening, and liquid biopsy. Typical image cytometry methods acquire two-dimensional (2D) fluorescence images, where the fluorescence labelling process may damage living cells, and the information from 2D images is not comprehensive enough for precise cell analysis. Although three-dimensional (3D) label-free image cytometry holds great promise, its high throughput development faces several technical challenges. Here, we report eXpress Single-frame CYtometer through Tomographic phasE (xSCYTE), which reconstructs 3D Refractive Index (RI) maps of cells with diffraction-limited resolution. With these high-speed and high-precision imaging capabilities empowered by artificial intelligence, we envision xSCYTE may open up many new avenues of biomedical investigations and industries, such as multi-omic assays and quality control during cellular therapeutic manufacturing. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2202_03627 |
| institution | arXiv |
| publishDate | 2022 |
| record_format | arxiv |
| spellingShingle | xSCYTE: Express Single-frame Cytometer through Tomographic Phase Ge, Baoliang He, Yanping Deng, Mo Rahman, Md Habibur Wang, Yijin Wu, Ziling Yang, Yongliang Kuang, Cuifang Wong, Chung Hong N. Chan, Michael K. Ho, Yi-Ping Duan, Liting Yaqoob, Zahid So, Peter T. C. Barbastathis, George Zhou, Renjie Optics Biological Physics Rapid, comprehensive, and accurate cell phenotyping without compromising viability, is crucial to many important biomedical applications, including stem-cell therapy, drug screening, and liquid biopsy. Typical image cytometry methods acquire two-dimensional (2D) fluorescence images, where the fluorescence labelling process may damage living cells, and the information from 2D images is not comprehensive enough for precise cell analysis. Although three-dimensional (3D) label-free image cytometry holds great promise, its high throughput development faces several technical challenges. Here, we report eXpress Single-frame CYtometer through Tomographic phasE (xSCYTE), which reconstructs 3D Refractive Index (RI) maps of cells with diffraction-limited resolution. With these high-speed and high-precision imaging capabilities empowered by artificial intelligence, we envision xSCYTE may open up many new avenues of biomedical investigations and industries, such as multi-omic assays and quality control during cellular therapeutic manufacturing. |
| title | xSCYTE: Express Single-frame Cytometer through Tomographic Phase |
| topic | Optics Biological Physics |
| url | https://arxiv.org/abs/2202.03627 |