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| Autori principali: | , , , , , , , |
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| Natura: | Preprint |
| Pubblicazione: |
2022
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| Soggetti: | |
| Accesso online: | https://arxiv.org/abs/2204.13068 |
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| _version_ | 1866909842289983488 |
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| author | Tran, Lan Hai Anh Lowe, Lauren A. Turner, Matthew Luong, James Khamis, Omar Abdullah A. Deckel, Yaam Amos, Megan L. Wang, Anna |
| author_facet | Tran, Lan Hai Anh Lowe, Lauren A. Turner, Matthew Luong, James Khamis, Omar Abdullah A. Deckel, Yaam Amos, Megan L. Wang, Anna |
| contents | We report efforts to quantify the loading of cell-sized lipid vesicles using in-line digital holographic microscopy. This method does not require fluorescent reporters, fluorescent tracers, or radioactive tracers. A single-color LED light source takes the place of conventional illumination to generate holograms rather than bright field images. By modelling the vesicle's scattering in a microscope with a Lorenz-Mie light scattering model, and comparing the results to data holograms, we are able to measure the vesicle's refractive index and thus loading. Performing the same comparison for bulk light scattering measurements enables retrieval of vesicle loading for nanoscale vesicles. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2204_13068 |
| institution | arXiv |
| publishDate | 2022 |
| record_format | arxiv |
| spellingShingle | Measuring vesicle loading with holographic microscopy and bulk light scattering Tran, Lan Hai Anh Lowe, Lauren A. Turner, Matthew Luong, James Khamis, Omar Abdullah A. Deckel, Yaam Amos, Megan L. Wang, Anna Soft Condensed Matter Biological Physics We report efforts to quantify the loading of cell-sized lipid vesicles using in-line digital holographic microscopy. This method does not require fluorescent reporters, fluorescent tracers, or radioactive tracers. A single-color LED light source takes the place of conventional illumination to generate holograms rather than bright field images. By modelling the vesicle's scattering in a microscope with a Lorenz-Mie light scattering model, and comparing the results to data holograms, we are able to measure the vesicle's refractive index and thus loading. Performing the same comparison for bulk light scattering measurements enables retrieval of vesicle loading for nanoscale vesicles. |
| title | Measuring vesicle loading with holographic microscopy and bulk light scattering |
| topic | Soft Condensed Matter Biological Physics |
| url | https://arxiv.org/abs/2204.13068 |