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Main Authors: Robertson, Connor, Wilmoth, Jared L., Retterer, Scott, Fuentes-Cabrera, Miguel
Format: Preprint
Published: 2022
Subjects:
Online Access:https://arxiv.org/abs/2205.05810
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author Robertson, Connor
Wilmoth, Jared L.
Retterer, Scott
Fuentes-Cabrera, Miguel
author_facet Robertson, Connor
Wilmoth, Jared L.
Retterer, Scott
Fuentes-Cabrera, Miguel
contents A Recurrent Neural Network (RNN) was used to perform video frame prediction of microbial growth for a population of two mutants of Pseudomonas aeruginosa. The RNN was trained on videos of 20 frames that were acquired using fluorescence microscopy and microfluidics. The network predicted the last 10 frames of each video, and the accuracy's of the predictions was assessed by comparing raw images, population curves, and the number and size of individual colonies. Overall, we found the predictions to be accurate using this approach. The implications this result has on designing autonomous experiments in microbiology, and the steps that can be taken to make the predictions even more accurate, are discussed.
format Preprint
id arxiv_https___arxiv_org_abs_2205_05810
institution arXiv
publishDate 2022
record_format arxiv
spellingShingle Performing Video Frame Prediction of Microbial Growth with a Recurrent Neural Network
Robertson, Connor
Wilmoth, Jared L.
Retterer, Scott
Fuentes-Cabrera, Miguel
Machine Learning
A Recurrent Neural Network (RNN) was used to perform video frame prediction of microbial growth for a population of two mutants of Pseudomonas aeruginosa. The RNN was trained on videos of 20 frames that were acquired using fluorescence microscopy and microfluidics. The network predicted the last 10 frames of each video, and the accuracy's of the predictions was assessed by comparing raw images, population curves, and the number and size of individual colonies. Overall, we found the predictions to be accurate using this approach. The implications this result has on designing autonomous experiments in microbiology, and the steps that can be taken to make the predictions even more accurate, are discussed.
title Performing Video Frame Prediction of Microbial Growth with a Recurrent Neural Network
topic Machine Learning
url https://arxiv.org/abs/2205.05810