A simple method to reprogram the binding specificity of DNA-coated colloids that crystallize
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arXiv
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| Main Authors: | , , , , |
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| Format: | Preprint |
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2022
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| _version_ | 1866913037403815936 |
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| author | Moerman, Pepijn G. Fang, Huang Videbæk, Thomas E. Rogers, W. Benjamin Schulman, Rebecca |
| author_facet | Moerman, Pepijn G. Fang, Huang Videbæk, Thomas E. Rogers, W. Benjamin Schulman, Rebecca |
| contents | DNA-coated colloids can crystallize into a multitude of lattices, ranging from face-centered cubic to diamond and thereby contribute to our understanding of crystallization and open avenues to producing structures with useful photonic properties. Despite the broad potential design space of DNA-coated colloids, the design cycle for synthesizing DNA-coated particles is slow: preparing a particle with a new type of DNA sequence takes more than one day and requires custom-made and chemically modified DNA that typically takes the supplier over a month to synthesize. Here, we introduce a method to generate particles with custom sequences from a single feed stock in under an hour at ambient conditions. Our method appends new DNA domains onto the DNA grafted to colloidal particles based on a template that takes the supplier less than a week to produce. The resultant particles crystallize as readily and at the same temperature as those produced via direct chemical synthesis. Moreover, we show that particles coated with a single sequence can be converted into a variety of building blocks with differing specificities by appending different DNA sequences to them. This approach to DNA-coated particle preparation will make it practical to identify optimal and complex particle sequence designs and to expand the use of DNA-coated colloids to a much broader range of investigators and commercial entities. |
| format | Preprint |
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arxiv_https___arxiv_org_abs_2206_00952 |
| institution | arXiv |
| publishDate | 2022 |
| record_format | arxiv |
| spellingShingle | A simple method to reprogram the binding specificity of DNA-coated colloids that crystallize Moerman, Pepijn G. Fang, Huang Videbæk, Thomas E. Rogers, W. Benjamin Schulman, Rebecca Soft Condensed Matter DNA-coated colloids can crystallize into a multitude of lattices, ranging from face-centered cubic to diamond and thereby contribute to our understanding of crystallization and open avenues to producing structures with useful photonic properties. Despite the broad potential design space of DNA-coated colloids, the design cycle for synthesizing DNA-coated particles is slow: preparing a particle with a new type of DNA sequence takes more than one day and requires custom-made and chemically modified DNA that typically takes the supplier over a month to synthesize. Here, we introduce a method to generate particles with custom sequences from a single feed stock in under an hour at ambient conditions. Our method appends new DNA domains onto the DNA grafted to colloidal particles based on a template that takes the supplier less than a week to produce. The resultant particles crystallize as readily and at the same temperature as those produced via direct chemical synthesis. Moreover, we show that particles coated with a single sequence can be converted into a variety of building blocks with differing specificities by appending different DNA sequences to them. This approach to DNA-coated particle preparation will make it practical to identify optimal and complex particle sequence designs and to expand the use of DNA-coated colloids to a much broader range of investigators and commercial entities. |
| title | A simple method to reprogram the binding specificity of DNA-coated colloids that crystallize |
| topic | Soft Condensed Matter |
| url | https://arxiv.org/abs/2206.00952 |