Enabling Collagen Quantification on HE-stained Slides Through Stain Deconvolution and Restained HE-HES
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arXiv
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| Autori principali: | , , , , , |
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| Natura: | Preprint |
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2022
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| author | Balezo, Guillaume Bertram, Christof A. Tilmant, Cyprien Petit, Stéphanie Hadj, Saima Ben Fick, Rutger H. J. |
| author_facet | Balezo, Guillaume Bertram, Christof A. Tilmant, Cyprien Petit, Stéphanie Hadj, Saima Ben Fick, Rutger H. J. |
| contents | In histology, the presence of collagen in the extra-cellular matrix has both diagnostic and prognostic value for cancer malignancy, and can be highlighted by adding Saffron (S) to a routine Hematoxylin and Eosin (HE) staining. However, Saffron is not usually added because of the additional cost and because pathologists are accustomed to HE, with the exception of France-based laboratories. In this paper, we show that it is possible to quantify the collagen content from the HE image alone and to digitally create an HES image. To do so, we trained a UNet to predict the Saffron densities from HE images. We created a dataset of registered, restained HE-HES slides and we extracted the Saffron concentrations as ground truth using stain deconvolution on the HES images. Our model reached a Mean Absolute Error of 0.0668 $\pm$ 0.0002 (Saffron values between 0 and 1) on a 3-fold testing set. We hope our approach can aid in improving the clinical workflow while reducing reagent costs for laboratories. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2211_09566 |
| institution | arXiv |
| publishDate | 2022 |
| record_format | arxiv |
| spellingShingle | Enabling Collagen Quantification on HE-stained Slides Through Stain Deconvolution and Restained HE-HES Balezo, Guillaume Bertram, Christof A. Tilmant, Cyprien Petit, Stéphanie Hadj, Saima Ben Fick, Rutger H. J. Image and Video Processing Computer Vision and Pattern Recognition In histology, the presence of collagen in the extra-cellular matrix has both diagnostic and prognostic value for cancer malignancy, and can be highlighted by adding Saffron (S) to a routine Hematoxylin and Eosin (HE) staining. However, Saffron is not usually added because of the additional cost and because pathologists are accustomed to HE, with the exception of France-based laboratories. In this paper, we show that it is possible to quantify the collagen content from the HE image alone and to digitally create an HES image. To do so, we trained a UNet to predict the Saffron densities from HE images. We created a dataset of registered, restained HE-HES slides and we extracted the Saffron concentrations as ground truth using stain deconvolution on the HES images. Our model reached a Mean Absolute Error of 0.0668 $\pm$ 0.0002 (Saffron values between 0 and 1) on a 3-fold testing set. We hope our approach can aid in improving the clinical workflow while reducing reagent costs for laboratories. |
| title | Enabling Collagen Quantification on HE-stained Slides Through Stain Deconvolution and Restained HE-HES |
| topic | Image and Video Processing Computer Vision and Pattern Recognition |
| url | https://arxiv.org/abs/2211.09566 |