Predicting Rubisco:Linker Condensation from Titration in the Dilute Phase
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| Main Authors: | , , , , , , , , |
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| Format: | Preprint |
| Published: |
2023
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| _version_ | 1866914668344246272 |
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| author | Payne-Dwyer, Alex Kumar, Gaurav Barrett, James Gherman, Laura K. Hodgkinson, Michael Plevin, Michael Mackinder, Luke Leake, Mark C. Schaefer, Charley |
| author_facet | Payne-Dwyer, Alex Kumar, Gaurav Barrett, James Gherman, Laura K. Hodgkinson, Michael Plevin, Michael Mackinder, Luke Leake, Mark C. Schaefer, Charley |
| contents | The condensation of Rubisco holoenzymes and linker proteins into 'pyrenoids', a crucial super-charger of photosynthesis in algae, is qualitatively understood in terms of 'sticker-and-spacer' theory. We derive semi-analytical partition sums for small Rubisco:linker aggregates, which enable the calculation of both dilute-phase titration curves and dimerisation diagrams. By fitting the titration curves to Surface Plasmon Resonance and Single-Molecule Fluorescence Microscopy data, we extract the molecular properties needed to predict dimerisation diagrams. We use these to estimate typical concentrations for condensation, and successfully compare these to microscopy observations. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2301_05681 |
| institution | arXiv |
| publishDate | 2023 |
| record_format | arxiv |
| spellingShingle | Predicting Rubisco:Linker Condensation from Titration in the Dilute Phase Payne-Dwyer, Alex Kumar, Gaurav Barrett, James Gherman, Laura K. Hodgkinson, Michael Plevin, Michael Mackinder, Luke Leake, Mark C. Schaefer, Charley Soft Condensed Matter Biological Physics The condensation of Rubisco holoenzymes and linker proteins into 'pyrenoids', a crucial super-charger of photosynthesis in algae, is qualitatively understood in terms of 'sticker-and-spacer' theory. We derive semi-analytical partition sums for small Rubisco:linker aggregates, which enable the calculation of both dilute-phase titration curves and dimerisation diagrams. By fitting the titration curves to Surface Plasmon Resonance and Single-Molecule Fluorescence Microscopy data, we extract the molecular properties needed to predict dimerisation diagrams. We use these to estimate typical concentrations for condensation, and successfully compare these to microscopy observations. |
| title | Predicting Rubisco:Linker Condensation from Titration in the Dilute Phase |
| topic | Soft Condensed Matter Biological Physics |
| url | https://arxiv.org/abs/2301.05681 |