Coherent X-ray Scattering Reveals Nanoscale Fluctuations in Hydrated Proteins
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arXiv
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| Autori principali: | , , , , , , , , , , , , , , , , , |
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| Natura: | Preprint |
| Pubblicazione: |
2023
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| author | Bin, Maddalena Reiser, Mario Filianina, Mariia Berkowicz, Sharon Das, Sudipta Timmermann, Sonja Roseker, Wojciech Bauer, Robert Öström, Jonatan Karina, Aigerim Amann-Winkel, Katrin Ladd-Parada, Marjorie Westermeier, Fabian Sprung, Michael Möller, Johannes Lehmkühler, Felix Gutt, Christian Perakis, Fivos |
| author_facet | Bin, Maddalena Reiser, Mario Filianina, Mariia Berkowicz, Sharon Das, Sudipta Timmermann, Sonja Roseker, Wojciech Bauer, Robert Öström, Jonatan Karina, Aigerim Amann-Winkel, Katrin Ladd-Parada, Marjorie Westermeier, Fabian Sprung, Michael Möller, Johannes Lehmkühler, Felix Gutt, Christian Perakis, Fivos |
| contents | Hydrated proteins undergo a transition in the deeply supercooled regime, which is attributed to rapid changes in hydration water and protein structural dynamics. Here, we investigate the nanoscale stress relaxation in hydrated lysozyme proteins stimulated and probed by X-ray Photon Correlation Spectroscopy (XPCS). This approach allows us to access the nanoscale dynamic response in the deeply supercooled regime (T = 180 K) which is typically not accessible through equilibrium methods. The relaxation time constants exhibit Arrhenius temperature dependence upon cooling with a minimum in the Kohlrausch-Williams-Watts exponent at T = 227 K. The observed minimum is attributed to an increase in dynamical heterogeneity, which coincides with enhanced fluctuations observed in the two-time correlation functions and a maximum in the dynamic susceptibility quantified by the normalised variance $χ_T$. Our study provides new insights into X-ray stimulated stress relaxation and the underlying mechanisms behind spatio-temporal fluctuations in biological granular materials. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2301_11043 |
| institution | arXiv |
| publishDate | 2023 |
| record_format | arxiv |
| spellingShingle | Coherent X-ray Scattering Reveals Nanoscale Fluctuations in Hydrated Proteins Bin, Maddalena Reiser, Mario Filianina, Mariia Berkowicz, Sharon Das, Sudipta Timmermann, Sonja Roseker, Wojciech Bauer, Robert Öström, Jonatan Karina, Aigerim Amann-Winkel, Katrin Ladd-Parada, Marjorie Westermeier, Fabian Sprung, Michael Möller, Johannes Lehmkühler, Felix Gutt, Christian Perakis, Fivos Soft Condensed Matter Biological Physics Chemical Physics Hydrated proteins undergo a transition in the deeply supercooled regime, which is attributed to rapid changes in hydration water and protein structural dynamics. Here, we investigate the nanoscale stress relaxation in hydrated lysozyme proteins stimulated and probed by X-ray Photon Correlation Spectroscopy (XPCS). This approach allows us to access the nanoscale dynamic response in the deeply supercooled regime (T = 180 K) which is typically not accessible through equilibrium methods. The relaxation time constants exhibit Arrhenius temperature dependence upon cooling with a minimum in the Kohlrausch-Williams-Watts exponent at T = 227 K. The observed minimum is attributed to an increase in dynamical heterogeneity, which coincides with enhanced fluctuations observed in the two-time correlation functions and a maximum in the dynamic susceptibility quantified by the normalised variance $χ_T$. Our study provides new insights into X-ray stimulated stress relaxation and the underlying mechanisms behind spatio-temporal fluctuations in biological granular materials. |
| title | Coherent X-ray Scattering Reveals Nanoscale Fluctuations in Hydrated Proteins |
| topic | Soft Condensed Matter Biological Physics Chemical Physics |
| url | https://arxiv.org/abs/2301.11043 |