Calculating Point Spread Functions: Methods, Pitfalls and Solutions
Fuente:
arXiv
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| Autori principali: | , , , , , |
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| Natura: | Preprint |
| Pubblicazione: |
2023
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| _version_ | 1866916153326043136 |
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| author | Miora, Ratsimandresy Holinirina Dina Rohwer, Erich Kielhorn, Martin Sheppard, Colin J. R. Bosman, Gurthwin Heintzmann, R. |
| author_facet | Miora, Ratsimandresy Holinirina Dina Rohwer, Erich Kielhorn, Martin Sheppard, Colin J. R. Bosman, Gurthwin Heintzmann, R. |
| contents | The knowledge of the exact structure of the optical system PSF enables a high-quality image reconstruction in fluorescence microscopy. Accurate PSF models account for the vector nature of light and the phase and amplitude modifications. Most existing real-space-based PSF models fall into a sampling pitfall near the centre position, yielding to the violation of the energy conservation. In this work, we present novel, to the best of our knowledge, Fourier-based techniques for computing vector PSF and compare them to the state-of-the-art. Our methods are shown to satisfy the physical condition of the imaging process. They are reproducible, computationally efficient, and easy to implement and easy to modify to represent various imaging modalities. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2301_13515 |
| institution | arXiv |
| publishDate | 2023 |
| record_format | arxiv |
| spellingShingle | Calculating Point Spread Functions: Methods, Pitfalls and Solutions Miora, Ratsimandresy Holinirina Dina Rohwer, Erich Kielhorn, Martin Sheppard, Colin J. R. Bosman, Gurthwin Heintzmann, R. Optics The knowledge of the exact structure of the optical system PSF enables a high-quality image reconstruction in fluorescence microscopy. Accurate PSF models account for the vector nature of light and the phase and amplitude modifications. Most existing real-space-based PSF models fall into a sampling pitfall near the centre position, yielding to the violation of the energy conservation. In this work, we present novel, to the best of our knowledge, Fourier-based techniques for computing vector PSF and compare them to the state-of-the-art. Our methods are shown to satisfy the physical condition of the imaging process. They are reproducible, computationally efficient, and easy to implement and easy to modify to represent various imaging modalities. |
| title | Calculating Point Spread Functions: Methods, Pitfalls and Solutions |
| topic | Optics |
| url | https://arxiv.org/abs/2301.13515 |