Timed material self-assembly controlled by circadian clock proteins
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arXiv
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| Autores principales: | , , , , , , , , , , , , |
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| Formato: | Preprint |
| Publicado: |
2023
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| _version_ | 1866916168742207488 |
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| author | Leech, Gregor Melcher, Lauren Chiu, Michelle Nugent, Maya Burton, Lily Kang, Janet Kim, Soo Ji Roy, Sourav Farhadi, Leila Ross, Jennifer L. Das, Moumita Rust, Michael J. Robertson-Anderson, Rae M. |
| author_facet | Leech, Gregor Melcher, Lauren Chiu, Michelle Nugent, Maya Burton, Lily Kang, Janet Kim, Soo Ji Roy, Sourav Farhadi, Leila Ross, Jennifer L. Das, Moumita Rust, Michael J. Robertson-Anderson, Rae M. |
| contents | Active biological molecules present a powerful, yet largely untapped, opportunity to impart autonomous regulation to materials. Because these systems can function robustly to regulate when and where chemical reactions occur, they have the ability to bring complex, life-like behavior to synthetic materials. Here, we achieve this design feat by using functionalized circadian clock proteins, KaiB and KaiC, to engineer time-dependent crosslinking of colloids. The resulting material self-assembles with programmable kinetics, producing macroscopic changes in material properties, via molecular assembly of KaiB-KaiC complexes. We show that colloid crosslinking depends strictly on the phosphorylation state of KaiC, with kinetics that are synced with KaiB-KaiC complexing. Our microscopic image analyses and computational models indicate that the stability of colloidal super-structures depends sensitively on the number of Kai complexes per colloid connection. Consistent with our model predictions, a high concentration stabilizes the material against dissolution after a robust self-assembly phase, while a low concentration allows circadian oscillation of material structure. This work introduces the concept of harnessing biological timers to control synthetic materials; and, more generally, opens the door to using protein-based reaction networks to endow synthetic systems with life-like functional properties. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2303_00779 |
| institution | arXiv |
| publishDate | 2023 |
| record_format | arxiv |
| spellingShingle | Timed material self-assembly controlled by circadian clock proteins Leech, Gregor Melcher, Lauren Chiu, Michelle Nugent, Maya Burton, Lily Kang, Janet Kim, Soo Ji Roy, Sourav Farhadi, Leila Ross, Jennifer L. Das, Moumita Rust, Michael J. Robertson-Anderson, Rae M. Soft Condensed Matter Biological Physics Active biological molecules present a powerful, yet largely untapped, opportunity to impart autonomous regulation to materials. Because these systems can function robustly to regulate when and where chemical reactions occur, they have the ability to bring complex, life-like behavior to synthetic materials. Here, we achieve this design feat by using functionalized circadian clock proteins, KaiB and KaiC, to engineer time-dependent crosslinking of colloids. The resulting material self-assembles with programmable kinetics, producing macroscopic changes in material properties, via molecular assembly of KaiB-KaiC complexes. We show that colloid crosslinking depends strictly on the phosphorylation state of KaiC, with kinetics that are synced with KaiB-KaiC complexing. Our microscopic image analyses and computational models indicate that the stability of colloidal super-structures depends sensitively on the number of Kai complexes per colloid connection. Consistent with our model predictions, a high concentration stabilizes the material against dissolution after a robust self-assembly phase, while a low concentration allows circadian oscillation of material structure. This work introduces the concept of harnessing biological timers to control synthetic materials; and, more generally, opens the door to using protein-based reaction networks to endow synthetic systems with life-like functional properties. |
| title | Timed material self-assembly controlled by circadian clock proteins |
| topic | Soft Condensed Matter Biological Physics |
| url | https://arxiv.org/abs/2303.00779 |