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Main Authors: Najar, Ulysse, Barolle, Victor, Balondrade, Paul, Fink, Mathias, Boccara, A. Claude, Aubry, Alexandre
Format: Preprint
Published: 2023
Subjects:
Online Access:https://arxiv.org/abs/2303.06119
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author Najar, Ulysse
Barolle, Victor
Balondrade, Paul
Fink, Mathias
Boccara, A. Claude
Aubry, Alexandre
author_facet Najar, Ulysse
Barolle, Victor
Balondrade, Paul
Fink, Mathias
Boccara, A. Claude
Aubry, Alexandre
contents As light travels through a disordered medium such as biological tissues, it undergoes multiple scattering events. This phenomenon is detrimental to in-depth optical microscopy, as it causes a drastic degradation of contrast, resolution and brightness of the resulting image beyond a few scattering mean free paths. However, the information about the inner reflectivity of the sample is not lost; only scrambled. To recover this information, a matrix approach of optical imaging can be fruitful. Here, we report on a de-scanned measurement of a high-dimension reflection matrix R via low coherence interferometry. Then, we show how a set of independent focusing laws can be extracted for each medium voxel through an iterative multi-scale analysis of wave distortions contained in R. It enables an optimal and local compensation of forward multiple scattering paths and provides a three-dimensional confocal image of the sample as the latter one had become digitally transparent. The proof-of-concept experiment is performed on a human opaque cornea and an extension of the penetration depth by a factor five is demonstrated compared to the state-of-the-art.
format Preprint
id arxiv_https___arxiv_org_abs_2303_06119
institution arXiv
publishDate 2023
record_format arxiv
spellingShingle Harnessing Forward Multiple Scattering for Optical Imaging Deep Inside an Opaque Medium
Najar, Ulysse
Barolle, Victor
Balondrade, Paul
Fink, Mathias
Boccara, A. Claude
Aubry, Alexandre
Optics
As light travels through a disordered medium such as biological tissues, it undergoes multiple scattering events. This phenomenon is detrimental to in-depth optical microscopy, as it causes a drastic degradation of contrast, resolution and brightness of the resulting image beyond a few scattering mean free paths. However, the information about the inner reflectivity of the sample is not lost; only scrambled. To recover this information, a matrix approach of optical imaging can be fruitful. Here, we report on a de-scanned measurement of a high-dimension reflection matrix R via low coherence interferometry. Then, we show how a set of independent focusing laws can be extracted for each medium voxel through an iterative multi-scale analysis of wave distortions contained in R. It enables an optimal and local compensation of forward multiple scattering paths and provides a three-dimensional confocal image of the sample as the latter one had become digitally transparent. The proof-of-concept experiment is performed on a human opaque cornea and an extension of the penetration depth by a factor five is demonstrated compared to the state-of-the-art.
title Harnessing Forward Multiple Scattering for Optical Imaging Deep Inside an Opaque Medium
topic Optics
url https://arxiv.org/abs/2303.06119