Laboratory-Based Correlative Soft X-ray and Fluorescence Microscopy in an Integrated Setup
Fuente:
arXiv
Saved in:
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Preprint |
| Published: |
2023
|
| Subjects: | |
| Online Access: | |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1866910306711633920 |
|---|---|
| author | Reinhard, Julius Kaleta, Sophia Abel, Johann Jakob Wiesner, Felix Wünsche, Martin Seemann, Eric Westermann, Martin Weber, Thomas Nathanael, Jan Iliou, Alexander Fiedorowicz, Henryk Hillmann, Falk Eggeling, Christian Paulus, Gerhard G. Fuchs, Silvio |
| author_facet | Reinhard, Julius Kaleta, Sophia Abel, Johann Jakob Wiesner, Felix Wünsche, Martin Seemann, Eric Westermann, Martin Weber, Thomas Nathanael, Jan Iliou, Alexander Fiedorowicz, Henryk Hillmann, Falk Eggeling, Christian Paulus, Gerhard G. Fuchs, Silvio |
| contents | Correlative microscopy is a powerful technique that combines the advantages of multiple imaging modalities to achieve a comprehensive understanding of investigated samples. For example, fluorescence microscopy provides unique functional contrast by imaging only specifically labeled components, especially in biological samples. However, the achievable structural information on the sample in its full complexity is limited. Here, the intrinsic label-free carbon contrast of water window soft X-ray microscopy can complement fluorescence images in a correlative approach ultimately combining nanoscale structural resolution with functional contrast. However, soft X-ray microscopes are complex and elaborate, and typically require a large-scale synchrotron radiation source due to the demanding photon flux requirements. Yet, with modern high-power lasers it has become possible to generate sufficient photon flux from laser-produced plasmas, thus enabling laboratory-based setups. Here, we present a compact table-top soft X-ray microscope with an integrated epifluorescence modality for 'in-situ' correlative imaging. Samples remain in place when switching between modalities, ensuring identical measurement conditions and avoiding sample alteration or destruction. We demonstrate our new method by multimodal images of several exemplary samples ranging from nanoparticles to various multicolor labeled cell types. A structural resolution of down to 50 nm was reached. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2304_14413 |
| institution | arXiv |
| publishDate | 2023 |
| record_format | arxiv |
| spellingShingle | Laboratory-Based Correlative Soft X-ray and Fluorescence Microscopy in an Integrated Setup Reinhard, Julius Kaleta, Sophia Abel, Johann Jakob Wiesner, Felix Wünsche, Martin Seemann, Eric Westermann, Martin Weber, Thomas Nathanael, Jan Iliou, Alexander Fiedorowicz, Henryk Hillmann, Falk Eggeling, Christian Paulus, Gerhard G. Fuchs, Silvio Instrumentation and Detectors Optics Correlative microscopy is a powerful technique that combines the advantages of multiple imaging modalities to achieve a comprehensive understanding of investigated samples. For example, fluorescence microscopy provides unique functional contrast by imaging only specifically labeled components, especially in biological samples. However, the achievable structural information on the sample in its full complexity is limited. Here, the intrinsic label-free carbon contrast of water window soft X-ray microscopy can complement fluorescence images in a correlative approach ultimately combining nanoscale structural resolution with functional contrast. However, soft X-ray microscopes are complex and elaborate, and typically require a large-scale synchrotron radiation source due to the demanding photon flux requirements. Yet, with modern high-power lasers it has become possible to generate sufficient photon flux from laser-produced plasmas, thus enabling laboratory-based setups. Here, we present a compact table-top soft X-ray microscope with an integrated epifluorescence modality for 'in-situ' correlative imaging. Samples remain in place when switching between modalities, ensuring identical measurement conditions and avoiding sample alteration or destruction. We demonstrate our new method by multimodal images of several exemplary samples ranging from nanoparticles to various multicolor labeled cell types. A structural resolution of down to 50 nm was reached. |
| title | Laboratory-Based Correlative Soft X-ray and Fluorescence Microscopy in an Integrated Setup |
| topic | Instrumentation and Detectors Optics |
| url | https://arxiv.org/abs/2304.14413 |