Tensorial tomographic Fourier Ptychography with applications to muscle tissue imaging
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arXiv
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| Auteurs principaux: | , , , , , , , , , , , , , |
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| Format: | Preprint |
| Publié: |
2023
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| _version_ | 1866913257392963584 |
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| author | Xu, Shiqi Dai, Xiang Ritter, Paul Lee, Kyung Chul Yang, Xi Kreiss, Lucas Zhou, Kevin C. Kim, Kanghyun Chaware, Amey Neff, Jadee Glass, Carolyn Lee, Seung Ah Friedrich, Oliver Horstmeyer, Roarke |
| author_facet | Xu, Shiqi Dai, Xiang Ritter, Paul Lee, Kyung Chul Yang, Xi Kreiss, Lucas Zhou, Kevin C. Kim, Kanghyun Chaware, Amey Neff, Jadee Glass, Carolyn Lee, Seung Ah Friedrich, Oliver Horstmeyer, Roarke |
| contents | We report Tensorial tomographic Fourier Ptychography (ToFu), a new non-scanning label-free tomographic microscopy method for simultaneous imaging of quantitative phase and anisotropic specimen information in 3D. Built upon Fourier Ptychography, a quantitative phase imaging technique, ToFu additionally highlights the vectorial nature of light. The imaging setup consists of a standard microscope equipped with an LED matrix, a polarization generator, and a polarization-sensitive camera. Permittivity tensors of anisotropic samples are computationally recovered from polarized intensity measurements across three dimensions. We demonstrate ToFu's efficiency through volumetric reconstructions of refractive index, birefringence, and orientation for various validation samples, as well as tissue samples from muscle fibers and diseased heart tissue. Our reconstructions of muscle fibers resolve their 3D fine-filament structure and yield consistent morphological measurements compared to gold-standard second harmonic generation scanning confocal microscope images found in the literature. Additionally, we demonstrate reconstructions of a heart tissue sample that carries important polarization information for detecting cardiac amyloidosis. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2305_05085 |
| institution | arXiv |
| publishDate | 2023 |
| record_format | arxiv |
| spellingShingle | Tensorial tomographic Fourier Ptychography with applications to muscle tissue imaging Xu, Shiqi Dai, Xiang Ritter, Paul Lee, Kyung Chul Yang, Xi Kreiss, Lucas Zhou, Kevin C. Kim, Kanghyun Chaware, Amey Neff, Jadee Glass, Carolyn Lee, Seung Ah Friedrich, Oliver Horstmeyer, Roarke Optics Image and Video Processing We report Tensorial tomographic Fourier Ptychography (ToFu), a new non-scanning label-free tomographic microscopy method for simultaneous imaging of quantitative phase and anisotropic specimen information in 3D. Built upon Fourier Ptychography, a quantitative phase imaging technique, ToFu additionally highlights the vectorial nature of light. The imaging setup consists of a standard microscope equipped with an LED matrix, a polarization generator, and a polarization-sensitive camera. Permittivity tensors of anisotropic samples are computationally recovered from polarized intensity measurements across three dimensions. We demonstrate ToFu's efficiency through volumetric reconstructions of refractive index, birefringence, and orientation for various validation samples, as well as tissue samples from muscle fibers and diseased heart tissue. Our reconstructions of muscle fibers resolve their 3D fine-filament structure and yield consistent morphological measurements compared to gold-standard second harmonic generation scanning confocal microscope images found in the literature. Additionally, we demonstrate reconstructions of a heart tissue sample that carries important polarization information for detecting cardiac amyloidosis. |
| title | Tensorial tomographic Fourier Ptychography with applications to muscle tissue imaging |
| topic | Optics Image and Video Processing |
| url | https://arxiv.org/abs/2305.05085 |