Demixing fluorescence time traces transmitted by multimode fibers
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arXiv
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| Autori principali: | , , , , , |
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| Natura: | Preprint |
| Pubblicazione: |
2023
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| _version_ | 1866910509084704768 |
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| author | Rimoli, Caio Vaz Moretti, Claudio Soldevila, Fernando Brémont, Enora Ventalon, Cathie Gigan, Sylvain |
| author_facet | Rimoli, Caio Vaz Moretti, Claudio Soldevila, Fernando Brémont, Enora Ventalon, Cathie Gigan, Sylvain |
| contents | Optical methods based on thin multimode fibers (MMFs) are promising tools for measuring neuronal activity in deep brain regions of freely moving mice thanks to their small diameter. However, current methods are limited: while fiber photometry provides only ensemble activity, imaging techniques using of long multimode fibers are very sensitive to bending and have not been applied to unrestrained rodents yet. Here, we demonstrate the fundamentals of a new approach using a short MMF coupled to a miniscope. In proof-of-principle in vitro experiments, we disentangled spatio-temporal fluorescence signals from multiple fluorescent sources transmitted by a thin (200 μm) and short (8 mm) MMF, using a general unconstrained non-negative matrix factorization algorithm directly on the raw video data. Furthermore, we show that low-cost open-source miniscopes have sufficient sensitivity to image the same fluorescence patterns seen in our proof-of-principle experiment, suggesting a new avenue for novel minimally invasive deep brain studies using multimode fibers in freely behaving mice. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2306_00695 |
| institution | arXiv |
| publishDate | 2023 |
| record_format | arxiv |
| spellingShingle | Demixing fluorescence time traces transmitted by multimode fibers Rimoli, Caio Vaz Moretti, Claudio Soldevila, Fernando Brémont, Enora Ventalon, Cathie Gigan, Sylvain Optics Applied Physics Biological Physics Neurons and Cognition Optical methods based on thin multimode fibers (MMFs) are promising tools for measuring neuronal activity in deep brain regions of freely moving mice thanks to their small diameter. However, current methods are limited: while fiber photometry provides only ensemble activity, imaging techniques using of long multimode fibers are very sensitive to bending and have not been applied to unrestrained rodents yet. Here, we demonstrate the fundamentals of a new approach using a short MMF coupled to a miniscope. In proof-of-principle in vitro experiments, we disentangled spatio-temporal fluorescence signals from multiple fluorescent sources transmitted by a thin (200 μm) and short (8 mm) MMF, using a general unconstrained non-negative matrix factorization algorithm directly on the raw video data. Furthermore, we show that low-cost open-source miniscopes have sufficient sensitivity to image the same fluorescence patterns seen in our proof-of-principle experiment, suggesting a new avenue for novel minimally invasive deep brain studies using multimode fibers in freely behaving mice. |
| title | Demixing fluorescence time traces transmitted by multimode fibers |
| topic | Optics Applied Physics Biological Physics Neurons and Cognition |
| url | https://arxiv.org/abs/2306.00695 |