Enregistré dans:
| Auteurs principaux: | , , , , , , , , , |
|---|---|
| Format: | Preprint |
| Publié: |
2023
|
| Sujets: | |
| Accès en ligne: | https://arxiv.org/abs/2306.05352 |
| Tags: |
Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!
|
| _version_ | 1866911916917522432 |
|---|---|
| author | Mudryk, Karen Lee, Chin Tomaník, Lukáš Malerz, Sebastian Trinter, Florian Hergenhahn, Uwe Neumark, Daniel M. Slavíček, Petr Bradforth, Stephen Winter, Bernd |
| author_facet | Mudryk, Karen Lee, Chin Tomaník, Lukáš Malerz, Sebastian Trinter, Florian Hergenhahn, Uwe Neumark, Daniel M. Slavíček, Petr Bradforth, Stephen Winter, Bernd |
| contents | Site-specific information on how adenosine triphosphate in the aqueous phase (ATP$_{(aq)}$) interacts with magnesium (Mg$^{2+}_{(aq)}$) is a prerequisite to understanding its complex biochemistry. To gather such information, we apply liquid-jet photoelectron spectroscopy (LJ-PES) assisted by electronic-structure calculations to study ATP$_{(aq)}$ solutions with and without dissolved Mg$^{2+}$. Valence photoemission data reveal spectral changes in the phosphate and adenine features of ATP$_{(aq)}$ due to interactions with the divalent cation. Chemical shifts in Mg 2p, Mg 2s, P 2p, and P 2s core-level spectra as a function of the Mg$^{2+}$/ATP concentration ratio are correlated to the formation of [MgATP]$^{-2}_{(aq)}$ and Mg$_2$ATP$_{(aq)}$ complexes, demonstrating the element-sensitivity of the technique to Mg$^{2+}$-phosphate interactions. In addition, we report and compare P 2s data from ATP$_{(aq)}$ and adenosine mono- and di-phosphate (AMP$_{(aq)}$ and ADP$_{(aq)}$, respectively) solutions, probing the electronic structure of the phosphate chain and the local environment of individual phosphate units in ATP$_{(aq)}$. Finally, we have recorded intermolecular Coulombic decay (ICD) spectra initiated by ionization of Mg 1s electrons to probe ligand exchange in the Mg$^{2+}$-ATP$_{(aq)}$ coordination environment, demonstrating the unique capabilities of ICD for revealing structural information. Our results provide an overview of the electronic structure of ATP$_{(aq)}$ and Mg$^{2+}$-ATP$_{(aq)}$ moieties relevant to phosphorylation and dephosphorylation reactions that are central to bioenergetics in living organisms. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2306_05352 |
| institution | arXiv |
| publishDate | 2023 |
| record_format | arxiv |
| spellingShingle | How Does Mg$^{2+}_{(aq)}$ Interact with ATP$_{(aq)}$? Biomolecular Structure through the Lens of Liquid-Jet Photoelectron Spectroscopy Mudryk, Karen Lee, Chin Tomaník, Lukáš Malerz, Sebastian Trinter, Florian Hergenhahn, Uwe Neumark, Daniel M. Slavíček, Petr Bradforth, Stephen Winter, Bernd Chemical Physics Site-specific information on how adenosine triphosphate in the aqueous phase (ATP$_{(aq)}$) interacts with magnesium (Mg$^{2+}_{(aq)}$) is a prerequisite to understanding its complex biochemistry. To gather such information, we apply liquid-jet photoelectron spectroscopy (LJ-PES) assisted by electronic-structure calculations to study ATP$_{(aq)}$ solutions with and without dissolved Mg$^{2+}$. Valence photoemission data reveal spectral changes in the phosphate and adenine features of ATP$_{(aq)}$ due to interactions with the divalent cation. Chemical shifts in Mg 2p, Mg 2s, P 2p, and P 2s core-level spectra as a function of the Mg$^{2+}$/ATP concentration ratio are correlated to the formation of [MgATP]$^{-2}_{(aq)}$ and Mg$_2$ATP$_{(aq)}$ complexes, demonstrating the element-sensitivity of the technique to Mg$^{2+}$-phosphate interactions. In addition, we report and compare P 2s data from ATP$_{(aq)}$ and adenosine mono- and di-phosphate (AMP$_{(aq)}$ and ADP$_{(aq)}$, respectively) solutions, probing the electronic structure of the phosphate chain and the local environment of individual phosphate units in ATP$_{(aq)}$. Finally, we have recorded intermolecular Coulombic decay (ICD) spectra initiated by ionization of Mg 1s electrons to probe ligand exchange in the Mg$^{2+}$-ATP$_{(aq)}$ coordination environment, demonstrating the unique capabilities of ICD for revealing structural information. Our results provide an overview of the electronic structure of ATP$_{(aq)}$ and Mg$^{2+}$-ATP$_{(aq)}$ moieties relevant to phosphorylation and dephosphorylation reactions that are central to bioenergetics in living organisms. |
| title | How Does Mg$^{2+}_{(aq)}$ Interact with ATP$_{(aq)}$? Biomolecular Structure through the Lens of Liquid-Jet Photoelectron Spectroscopy |
| topic | Chemical Physics |
| url | https://arxiv.org/abs/2306.05352 |