Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Preprint |
| Published: |
2023
|
| Subjects: | |
| Online Access: | https://arxiv.org/abs/2311.04674 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1866929310116347904 |
|---|---|
| author | Jaric, Stefan Schobesberger, Silvia Velicki, Lazar Milovancev, Aleksandra Nikolic, Stanislava Ertl, Peter Bobrinetskiy, Ivan Knezevic, Nikola Z. |
| author_facet | Jaric, Stefan Schobesberger, Silvia Velicki, Lazar Milovancev, Aleksandra Nikolic, Stanislava Ertl, Peter Bobrinetskiy, Ivan Knezevic, Nikola Z. |
| contents | Simple and low-cost biosensing solutions are suitable for point-of-care applications aiming to overcome the gap between scientific concepts and technological production. To compete with sensitivity and selectivity of golden standards, such as liquid chromatography, the functionalization of biosensors is continuously optimized to enhance the signal and improve their performance, often leading to complex chemical assay development. In this research, the efforts are made on optimizing the methodology for electrochemical reduction of graphene oxide to produce thin film-modified gold electrodes. Under the employed specific conditions, 20 cycles of cyclic voltammetry (CV) are shown to be optimal for superior electrical activation of graphene oxide into electrochemically reduced graphene oxide (ERGO). This platform is further used to develop a matrix metalloproteinase 2 (MMP-2) biosensor, where specific anti-MMP2 aptamers are utilized as a biorecognition element. MMP-2 is a protein which is typically overexpressed in tumor tissues, with important roles in tumor invasion, metastasis as well as in tumor angiogenesis. Based on impedimetric measurements, we were able to detect as low as 3.32 pg/mL of MMP-2 in PBS with a dynamic range of 10 pg/mL - 10 ng/mL. Besides high specificity, ERGO-based aptasensor showed a potential of reuse due to demonstrated successful signal restoration after experimental detection of MMP-2. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2311_04674 |
| institution | arXiv |
| publishDate | 2023 |
| record_format | arxiv |
| spellingShingle | Direct electrochemical reduction of graphene oxide thin film for aptamer-based selective and highly sensitive detection of Matrix metalloproteinase 2 Jaric, Stefan Schobesberger, Silvia Velicki, Lazar Milovancev, Aleksandra Nikolic, Stanislava Ertl, Peter Bobrinetskiy, Ivan Knezevic, Nikola Z. Applied Physics Instrumentation and Detectors Biomolecules Simple and low-cost biosensing solutions are suitable for point-of-care applications aiming to overcome the gap between scientific concepts and technological production. To compete with sensitivity and selectivity of golden standards, such as liquid chromatography, the functionalization of biosensors is continuously optimized to enhance the signal and improve their performance, often leading to complex chemical assay development. In this research, the efforts are made on optimizing the methodology for electrochemical reduction of graphene oxide to produce thin film-modified gold electrodes. Under the employed specific conditions, 20 cycles of cyclic voltammetry (CV) are shown to be optimal for superior electrical activation of graphene oxide into electrochemically reduced graphene oxide (ERGO). This platform is further used to develop a matrix metalloproteinase 2 (MMP-2) biosensor, where specific anti-MMP2 aptamers are utilized as a biorecognition element. MMP-2 is a protein which is typically overexpressed in tumor tissues, with important roles in tumor invasion, metastasis as well as in tumor angiogenesis. Based on impedimetric measurements, we were able to detect as low as 3.32 pg/mL of MMP-2 in PBS with a dynamic range of 10 pg/mL - 10 ng/mL. Besides high specificity, ERGO-based aptasensor showed a potential of reuse due to demonstrated successful signal restoration after experimental detection of MMP-2. |
| title | Direct electrochemical reduction of graphene oxide thin film for aptamer-based selective and highly sensitive detection of Matrix metalloproteinase 2 |
| topic | Applied Physics Instrumentation and Detectors Biomolecules |
| url | https://arxiv.org/abs/2311.04674 |