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Main Authors: Ottino-Loffler, Bertrand, Victora, Gabriel
Format: Preprint
Published: 2023
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Online Access:https://arxiv.org/abs/2312.05785
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author Ottino-Loffler, Bertrand
Victora, Gabriel
author_facet Ottino-Loffler, Bertrand
Victora, Gabriel
contents A central feature of vertebrate immune response is affinity maturation, wherein antibody-producing B cells undergo evolutionary selection in microanatomical structures called germinal centers, which form in secondary lymphoid organs upon antigen exposure. While it has been shown that the median B cell affinity dependably increases over the course of maturation, the exact logic behind this evolution remains vague. Three potential selection methods include encouraging the reproduction of high affinity cells (``birth/positive selection''), encouraging cell death in low affinity cells (``death/negative selection''), and adjusting the mutation rate based on cell affinity (``mutational selection''). While all three forms of selection would lead to a net increase in affinity, different selection methods may lead to distinct statistical dynamics. We present a tractable model of selection, and analyze proposed signatures of negative selection. Given the simplicity of the model, such signatures should be stronger here than in real systems. However, we find a number of intuitively appealing metrics -- such as preferential ancestry ratios, terminal node counts, and mutation count skewness -- are all ill-suited for detecting selection method.
format Preprint
id arxiv_https___arxiv_org_abs_2312_05785
institution arXiv
publishDate 2023
record_format arxiv
spellingShingle On Possible Indicators of Negative Selection in Germinal Centers
Ottino-Loffler, Bertrand
Victora, Gabriel
Populations and Evolution
Dynamical Systems
A central feature of vertebrate immune response is affinity maturation, wherein antibody-producing B cells undergo evolutionary selection in microanatomical structures called germinal centers, which form in secondary lymphoid organs upon antigen exposure. While it has been shown that the median B cell affinity dependably increases over the course of maturation, the exact logic behind this evolution remains vague. Three potential selection methods include encouraging the reproduction of high affinity cells (``birth/positive selection''), encouraging cell death in low affinity cells (``death/negative selection''), and adjusting the mutation rate based on cell affinity (``mutational selection''). While all three forms of selection would lead to a net increase in affinity, different selection methods may lead to distinct statistical dynamics. We present a tractable model of selection, and analyze proposed signatures of negative selection. Given the simplicity of the model, such signatures should be stronger here than in real systems. However, we find a number of intuitively appealing metrics -- such as preferential ancestry ratios, terminal node counts, and mutation count skewness -- are all ill-suited for detecting selection method.
title On Possible Indicators of Negative Selection in Germinal Centers
topic Populations and Evolution
Dynamical Systems
url https://arxiv.org/abs/2312.05785