Super-Resolution Microscopy Based on the Inherent Fluctuations of Dye Molecules
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arXiv
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| Autores principales: | , , , , , |
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| Formato: | Preprint |
| Publicado: |
2023
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| _version_ | 1866929450982047744 |
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| author | Krupinski-Ptaszek, Alexander Makowski, Adrian Mielnicka, Aleksandra Pawłowska, Monika Tenne, Ron Lapkiewicz, Radek |
| author_facet | Krupinski-Ptaszek, Alexander Makowski, Adrian Mielnicka, Aleksandra Pawłowska, Monika Tenne, Ron Lapkiewicz, Radek |
| contents | Fluorescence microscopy is a critical tool across various disciplines, from materials science to biomedical research, yet it is limited by the diffraction limit of resolution. Advanced super-resolution techniques such as localization microscopy and stimulated-emission-depletion microscopy often demand considerable resources. These methods depend heavily on elaborate sample-staining, complex optical systems, or prolonged acquisition periods, and their application in 3D and multicolor imaging presents significant experimental challenges. In the current work, we provide a complete demonstration of a widely accessible super-resolution imaging approach capable of 3D and multicolor imaging. We replace the confocal pinhole with an array of single-photon avalanche diodes and use the microsecond-scale fluctuations of dye molecules as a contrast mechanism. This contrast is transformed into a super-resolved image using a robust and deterministic algorithm. Our technique utilizes natural fluctuations inherent to organic dyes, thereby it does not require engineering of the blinking statistics. Our robust, versatile super-resolution method opens the way to next-generation multimodal imaging and facilitates on-demand super-resolution within a confocal architecture. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2401_00261 |
| institution | arXiv |
| publishDate | 2023 |
| record_format | arxiv |
| spellingShingle | Super-Resolution Microscopy Based on the Inherent Fluctuations of Dye Molecules Krupinski-Ptaszek, Alexander Makowski, Adrian Mielnicka, Aleksandra Pawłowska, Monika Tenne, Ron Lapkiewicz, Radek Optics Biological Physics Fluorescence microscopy is a critical tool across various disciplines, from materials science to biomedical research, yet it is limited by the diffraction limit of resolution. Advanced super-resolution techniques such as localization microscopy and stimulated-emission-depletion microscopy often demand considerable resources. These methods depend heavily on elaborate sample-staining, complex optical systems, or prolonged acquisition periods, and their application in 3D and multicolor imaging presents significant experimental challenges. In the current work, we provide a complete demonstration of a widely accessible super-resolution imaging approach capable of 3D and multicolor imaging. We replace the confocal pinhole with an array of single-photon avalanche diodes and use the microsecond-scale fluctuations of dye molecules as a contrast mechanism. This contrast is transformed into a super-resolved image using a robust and deterministic algorithm. Our technique utilizes natural fluctuations inherent to organic dyes, thereby it does not require engineering of the blinking statistics. Our robust, versatile super-resolution method opens the way to next-generation multimodal imaging and facilitates on-demand super-resolution within a confocal architecture. |
| title | Super-Resolution Microscopy Based on the Inherent Fluctuations of Dye Molecules |
| topic | Optics Biological Physics |
| url | https://arxiv.org/abs/2401.00261 |