Live-cell imaging powered by computation
Fuente:
arXiv
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| Auteurs principaux: | , , , |
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| Format: | Preprint |
| Publié: |
2024
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| _version_ | 1866929196540887040 |
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| author | Shroff, Hari Testa, Ilaria Jug, Florian Manley, Suliana |
| author_facet | Shroff, Hari Testa, Ilaria Jug, Florian Manley, Suliana |
| contents | The proliferation of microscopy methods for live-cell imaging offers many new possibilities for users but can also be challenging to navigate. We focus here on computational methods that promise to boost live-cell fluorescence microscopy, where intra-cellular dynamics and cell viability constrain measurements considerably. Considering the tradeoffs between signal-to-noise ratio (SNR), spatial resolution, temporal resolution, and multi-color and multi-channel capacity, we review computational methods that can be layered on top of commonly used existing microscopies, as well as hybrid methods that integrate computation and microscope hardware. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2401_01438 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | Live-cell imaging powered by computation Shroff, Hari Testa, Ilaria Jug, Florian Manley, Suliana Quantitative Methods Biological Physics The proliferation of microscopy methods for live-cell imaging offers many new possibilities for users but can also be challenging to navigate. We focus here on computational methods that promise to boost live-cell fluorescence microscopy, where intra-cellular dynamics and cell viability constrain measurements considerably. Considering the tradeoffs between signal-to-noise ratio (SNR), spatial resolution, temporal resolution, and multi-color and multi-channel capacity, we review computational methods that can be layered on top of commonly used existing microscopies, as well as hybrid methods that integrate computation and microscope hardware. |
| title | Live-cell imaging powered by computation |
| topic | Quantitative Methods Biological Physics |
| url | https://arxiv.org/abs/2401.01438 |