Using polarization sensitive SMLM to infer the interaction strength of dye-plasmonic nanosphere systems
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arXiv
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| Auteurs principaux: | , , , , , , , |
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| Format: | Preprint |
| Publié: |
2024
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| _version_ | 1866917563565342720 |
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| author | Novák, T. Bíró, P. Ferenc, Gy. Ungor, D. Czvik, E. Deák, Á. Janovák, L. Erdélyi, M. |
| author_facet | Novák, T. Bíró, P. Ferenc, Gy. Ungor, D. Czvik, E. Deák, Á. Janovák, L. Erdélyi, M. |
| contents | Single molecule microscopy has proven to be an effective tool to characterize the fluorophore-plasmonic structure interaction. However, as specific information is hidden in the emission, sophisticated evaluation is required. Here we investigated the emission polarization of rotationally mobile fluorophores near plasmonic Au/Ag alloy nanospheres both theoretically and experimentally. Our work surpasses the results of the previous studies in that it considers the rotational mobility of the fluorophores attached to metallic nanoparticles. Through theoretical modeling and elaborate numerical calculations we determined the expected measurable fluorescence polarization, and via DNA-PAINT single molecule measurements we validated the theoretical predictions. Our findings suggest that through measuring the polarization state of a sequence of single-molecule events, it is possible to infer the interaction strength of the dye-nanosphere system. A precise description requires elaborate calculations, however, a simplified model is able to predict the tendency of the polarization state with the change of experimental parameters. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2401_05231 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | Using polarization sensitive SMLM to infer the interaction strength of dye-plasmonic nanosphere systems Novák, T. Bíró, P. Ferenc, Gy. Ungor, D. Czvik, E. Deák, Á. Janovák, L. Erdélyi, M. Optics Single molecule microscopy has proven to be an effective tool to characterize the fluorophore-plasmonic structure interaction. However, as specific information is hidden in the emission, sophisticated evaluation is required. Here we investigated the emission polarization of rotationally mobile fluorophores near plasmonic Au/Ag alloy nanospheres both theoretically and experimentally. Our work surpasses the results of the previous studies in that it considers the rotational mobility of the fluorophores attached to metallic nanoparticles. Through theoretical modeling and elaborate numerical calculations we determined the expected measurable fluorescence polarization, and via DNA-PAINT single molecule measurements we validated the theoretical predictions. Our findings suggest that through measuring the polarization state of a sequence of single-molecule events, it is possible to infer the interaction strength of the dye-nanosphere system. A precise description requires elaborate calculations, however, a simplified model is able to predict the tendency of the polarization state with the change of experimental parameters. |
| title | Using polarization sensitive SMLM to infer the interaction strength of dye-plasmonic nanosphere systems |
| topic | Optics |
| url | https://arxiv.org/abs/2401.05231 |