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Bibliographic Details
Main Authors: Krishna, Krishangi, Burrow, Joshua A., Jiang, Zhaowei, Liu, Wenyu, Shukla, Anita, Toussaint Jr, Kimani C.
Format: Preprint
Published: 2024
Subjects:
Online Access:https://arxiv.org/abs/2401.06906
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Table of Contents:
  • Traditional optical tweezers techniques often rely on high-power continuous wave (CW) lasers, which can introduce unwanted thermal effects and photodamage to delicate samples. To overcome these limitations, we demonstrate femtosecond laser assisted selective holding with ultra-low power (FLASH-UP). We find that the FLASH-UP exhibits a five times greater trap stiffness than CW-OT, and can trap at lower intensities. Furthermore, we demonstrate OT of different pathogenic bacteria species and find that FLASH-UP does not impact cell motility. These results pave the way for applications in sorting, bio-sensing, in vivo cell manipulation and single cell analysis.