Ultra-broadband Optical Diffraction Tomography
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arXiv
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| Autores principales: | , , , , |
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| Formato: | Preprint |
| Publicado: |
2024
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| _version_ | 1866910426016514048 |
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| author | Hörmann, Martin Camargo, Franco V. A. van Hulst, Niek F. Cerullo, Giulio Liebel, Matz |
| author_facet | Hörmann, Martin Camargo, Franco V. A. van Hulst, Niek F. Cerullo, Giulio Liebel, Matz |
| contents | Optical diffraction tomography (ODT) is a powerful non-invasive 3D imaging technique, but its combination with broadband light sources is difficult. In this study, we introduce ultrabroadband ODT, covering over 150 nm of visible spectral bandwidth with a lateral spatial resolution of 150 nm. Our work addresses a critical experimental gap by enabling the measurement of broadband refractive index changes in 3D samples, a crucial information that is difficult to assess with existing methodologies. We present broadband, spectrally resolved ODT images of HeLa cells, obtained via pulse-shaping based Fourier transform spectroscopy. The spectral observations enabled by ultrabroadband ODT, combined with material-dependent refractive index responses, allow for precise three-dimensional identification of the nanoparticles within cellular structures. Our work represents a crucial step towards time and spectrally-resolved tomography of complex 3D structures with implications for life and materials science applications. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2401_07391 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | Ultra-broadband Optical Diffraction Tomography Hörmann, Martin Camargo, Franco V. A. van Hulst, Niek F. Cerullo, Giulio Liebel, Matz Optics Biological Physics Optical diffraction tomography (ODT) is a powerful non-invasive 3D imaging technique, but its combination with broadband light sources is difficult. In this study, we introduce ultrabroadband ODT, covering over 150 nm of visible spectral bandwidth with a lateral spatial resolution of 150 nm. Our work addresses a critical experimental gap by enabling the measurement of broadband refractive index changes in 3D samples, a crucial information that is difficult to assess with existing methodologies. We present broadband, spectrally resolved ODT images of HeLa cells, obtained via pulse-shaping based Fourier transform spectroscopy. The spectral observations enabled by ultrabroadband ODT, combined with material-dependent refractive index responses, allow for precise three-dimensional identification of the nanoparticles within cellular structures. Our work represents a crucial step towards time and spectrally-resolved tomography of complex 3D structures with implications for life and materials science applications. |
| title | Ultra-broadband Optical Diffraction Tomography |
| topic | Optics Biological Physics |
| url | https://arxiv.org/abs/2401.07391 |