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| Main Authors: | , , , , , , |
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| Format: | Preprint |
| Published: |
2024
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| Subjects: | |
| Online Access: | https://arxiv.org/abs/2401.09085 |
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| _version_ | 1866910304169885696 |
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| author | Liu, Xiaowei Tang, Mingwei Zhou, Ning Pang, Chenlei Wen, Zhong Liu, Xu Yang, Qing |
| author_facet | Liu, Xiaowei Tang, Mingwei Zhou, Ning Pang, Chenlei Wen, Zhong Liu, Xu Yang, Qing |
| contents | Super-resolution mapping of the 3D orientation of fluorophores reveals the alignment of biological structures where the fluorophores are tightly attached, and thus plays a vital role in studying the organization and dynamics of bio-complexes. However, current super-resolution imaging techniques are either limited to 2D orientation mapping or suffer from slow speed and the requirement of special labels in 3D orientation mapping. Here, we propose a novel polarized virtual spatial-frequency-shift effect to overcome these restrictions to achieve a universal 3D orientation super-resolution mapping capability. To demonstrate the mechanism, we simulate the imaging process and reconstruct the spatial-angular information for sparsely distributed dipoles with random 3D orientations and microfilament-like structures decorated with fluorophores oriented parallel to them. The 3D orientation distribution can be recovered with a doubled spatial resolution and an average angular precision of up to 2.39 degrees. The performance of the approach with noise has also been analyzed considering real implementation. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2401_09085 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | 3D orientation super-resolution spatial-frequency-shift microscopy Liu, Xiaowei Tang, Mingwei Zhou, Ning Pang, Chenlei Wen, Zhong Liu, Xu Yang, Qing Optics Super-resolution mapping of the 3D orientation of fluorophores reveals the alignment of biological structures where the fluorophores are tightly attached, and thus plays a vital role in studying the organization and dynamics of bio-complexes. However, current super-resolution imaging techniques are either limited to 2D orientation mapping or suffer from slow speed and the requirement of special labels in 3D orientation mapping. Here, we propose a novel polarized virtual spatial-frequency-shift effect to overcome these restrictions to achieve a universal 3D orientation super-resolution mapping capability. To demonstrate the mechanism, we simulate the imaging process and reconstruct the spatial-angular information for sparsely distributed dipoles with random 3D orientations and microfilament-like structures decorated with fluorophores oriented parallel to them. The 3D orientation distribution can be recovered with a doubled spatial resolution and an average angular precision of up to 2.39 degrees. The performance of the approach with noise has also been analyzed considering real implementation. |
| title | 3D orientation super-resolution spatial-frequency-shift microscopy |
| topic | Optics |
| url | https://arxiv.org/abs/2401.09085 |