Ratiometric fluorescent sensing of pyrophosphate with sp$^{3}$-functionalized single-walled carbon nanotubes
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arXiv
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| Format: | Preprint |
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2024
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| author | Settele, Simon Schrage, C. Alexander Jung, Sebastian Michel, Elena Li, Han Flavel, Benjamin S. Hashmi, A. Stephen K. Kruss, Sebastian Zaumseil, Jana |
| author_facet | Settele, Simon Schrage, C. Alexander Jung, Sebastian Michel, Elena Li, Han Flavel, Benjamin S. Hashmi, A. Stephen K. Kruss, Sebastian Zaumseil, Jana |
| contents | Inorganic pyrophosphate is a key molecule in many biological processes from DNA synthesis to cell metabolism. Here we introduce sp$^{3}$-functionalized (6,5) single-walled carbon nanotubes (SWNTs) with red-shifted defect emission as near-infrared luminescent probes for the optical detection and quantification of inorganic pyrophosphate. The sensing scheme is based on the immobilization of Cu$^{2+}$ ions on the SWNT surface promoted by coordination to covalently attached aryl alkyne groups and a triazole complex. The presence of Cu$^{2+}$ ions on the SWNT surface causes fluorescence quenching via photoinduced electron transfer, which is reversed by copper-complexing analytes such as pyrophosphate. The differences in the fluorescence response of sp$^{3}$-defect to pristine nanotube emission enables reproducible ratiometric measurements in a wide concentration window. Biocompatible, phospholipid-polyethylene glycol-coated SWNTs with such sp$^{3}$ defects are employed for the detection of pyrophosphate in cell lysate and for monitoring the progress of DNA synthesis in a polymerase chain reaction. This robust ratiometric and near-infrared luminescent probe for pyrophosphate may serve as a starting point for the rational design of nanotube-based biosensors. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2401_17667 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | Ratiometric fluorescent sensing of pyrophosphate with sp$^{3}$-functionalized single-walled carbon nanotubes Settele, Simon Schrage, C. Alexander Jung, Sebastian Michel, Elena Li, Han Flavel, Benjamin S. Hashmi, A. Stephen K. Kruss, Sebastian Zaumseil, Jana Applied Physics Materials Science Inorganic pyrophosphate is a key molecule in many biological processes from DNA synthesis to cell metabolism. Here we introduce sp$^{3}$-functionalized (6,5) single-walled carbon nanotubes (SWNTs) with red-shifted defect emission as near-infrared luminescent probes for the optical detection and quantification of inorganic pyrophosphate. The sensing scheme is based on the immobilization of Cu$^{2+}$ ions on the SWNT surface promoted by coordination to covalently attached aryl alkyne groups and a triazole complex. The presence of Cu$^{2+}$ ions on the SWNT surface causes fluorescence quenching via photoinduced electron transfer, which is reversed by copper-complexing analytes such as pyrophosphate. The differences in the fluorescence response of sp$^{3}$-defect to pristine nanotube emission enables reproducible ratiometric measurements in a wide concentration window. Biocompatible, phospholipid-polyethylene glycol-coated SWNTs with such sp$^{3}$ defects are employed for the detection of pyrophosphate in cell lysate and for monitoring the progress of DNA synthesis in a polymerase chain reaction. This robust ratiometric and near-infrared luminescent probe for pyrophosphate may serve as a starting point for the rational design of nanotube-based biosensors. |
| title | Ratiometric fluorescent sensing of pyrophosphate with sp$^{3}$-functionalized single-walled carbon nanotubes |
| topic | Applied Physics Materials Science |
| url | https://arxiv.org/abs/2401.17667 |