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| Main Authors: | , , , , , , , , , , |
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| Format: | Preprint |
| Published: |
2024
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| Subjects: | |
| Online Access: | https://arxiv.org/abs/2402.02326 |
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| _version_ | 1866929236926791680 |
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| author | Wen, Junpeng Pilger, Christian Wang, Wenlong Erapaneedi, Raghu Xiu, Hao Fan, Yiheng Hu, Xu Huser, Thomas Kiefer, Friedemann Wei, Xiaoming Yang, Zhongmin |
| author_facet | Wen, Junpeng Pilger, Christian Wang, Wenlong Erapaneedi, Raghu Xiu, Hao Fan, Yiheng Hu, Xu Huser, Thomas Kiefer, Friedemann Wei, Xiaoming Yang, Zhongmin |
| contents | We demonstrate a compact watt-level all polarization-maintaining (PM) femtosecond fiber laser source at 1100 nm. The fiber laser source is seeded by an all PM fiber mode-locked laser employing a nonlinear amplifying loop mirror. The seed laser can generate stable pulses at a fundamental repetition rate of 40.71 MHz with a signal-to-noise rate of >100 dB and an integrated relative intensity noise of only ~0.061%. After two-stage external amplification and pulse compression, an output power of ~1.47 W (corresponding to a pulse energy of ~36.1 nJ) and a pulse duration of ~251 fs are obtained. The 1100 nm femtosecond fiber laser is then employed as the excitation light source for multicolor multi-photon fluorescence microscopy of Chinese hamster ovary (CHO) cells stably expressing red fluorescent proteins. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2402_02326 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | Watt-level all polarization-maintaining femtosecond fiber laser source at 1100 nm for multicolor two-photon fluorescence excitation of fluorescent proteins Wen, Junpeng Pilger, Christian Wang, Wenlong Erapaneedi, Raghu Xiu, Hao Fan, Yiheng Hu, Xu Huser, Thomas Kiefer, Friedemann Wei, Xiaoming Yang, Zhongmin Optics Biological Physics We demonstrate a compact watt-level all polarization-maintaining (PM) femtosecond fiber laser source at 1100 nm. The fiber laser source is seeded by an all PM fiber mode-locked laser employing a nonlinear amplifying loop mirror. The seed laser can generate stable pulses at a fundamental repetition rate of 40.71 MHz with a signal-to-noise rate of >100 dB and an integrated relative intensity noise of only ~0.061%. After two-stage external amplification and pulse compression, an output power of ~1.47 W (corresponding to a pulse energy of ~36.1 nJ) and a pulse duration of ~251 fs are obtained. The 1100 nm femtosecond fiber laser is then employed as the excitation light source for multicolor multi-photon fluorescence microscopy of Chinese hamster ovary (CHO) cells stably expressing red fluorescent proteins. |
| title | Watt-level all polarization-maintaining femtosecond fiber laser source at 1100 nm for multicolor two-photon fluorescence excitation of fluorescent proteins |
| topic | Optics Biological Physics |
| url | https://arxiv.org/abs/2402.02326 |