Unleashing Urease Protein Dynamics with Metamaterial Optical Tweezers
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arXiv
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| Auteurs principaux: | , , , , , |
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| Format: | Preprint |
| Publié: |
2024
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| _version_ | 1866912483528146944 |
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| author | Kotsifaki, Domna G. Truong, Viet Giang Dindo, Mirco Cichos, Frank Laurino, Paolo Chormaic, Sile Nic |
| author_facet | Kotsifaki, Domna G. Truong, Viet Giang Dindo, Mirco Cichos, Frank Laurino, Paolo Chormaic, Sile Nic |
| contents | Investigating the dynamics of single biomolecules is essential for unlocking new frontiers in biophysics and medicine. Here, we present a transformative approach using metamaterial optical tweezers to trap and study individual urease molecules - an enzyme that catalyzes urea hydrolysis and serves as a key biomarker for pathogenic infections. By generating thermally induced drifts and rapid hydrodynamic flows with optically induced local temperature fields at the metamaterial/water interface, we achieve unprecedented guidance and control over single urease molecules at low trapping laser intensities of 0.13~mW/μm^2 facilitating their precise delivery to plasmonic hotspots. The interplay of thermophoresis, thermo-osmosis and depletion forces shapes the trapping potential, enabling real-time observation of ureases` conformational changes and interactions with the environment. This innovative approach not only enhances our understanding of enzyme dynamics but also paves the way for label-free characterization of biomolecules, ushering in a new era in life sciences and nanotechnology. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2402_12878 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | Unleashing Urease Protein Dynamics with Metamaterial Optical Tweezers Kotsifaki, Domna G. Truong, Viet Giang Dindo, Mirco Cichos, Frank Laurino, Paolo Chormaic, Sile Nic Optics Biological Physics Investigating the dynamics of single biomolecules is essential for unlocking new frontiers in biophysics and medicine. Here, we present a transformative approach using metamaterial optical tweezers to trap and study individual urease molecules - an enzyme that catalyzes urea hydrolysis and serves as a key biomarker for pathogenic infections. By generating thermally induced drifts and rapid hydrodynamic flows with optically induced local temperature fields at the metamaterial/water interface, we achieve unprecedented guidance and control over single urease molecules at low trapping laser intensities of 0.13~mW/μm^2 facilitating their precise delivery to plasmonic hotspots. The interplay of thermophoresis, thermo-osmosis and depletion forces shapes the trapping potential, enabling real-time observation of ureases` conformational changes and interactions with the environment. This innovative approach not only enhances our understanding of enzyme dynamics but also paves the way for label-free characterization of biomolecules, ushering in a new era in life sciences and nanotechnology. |
| title | Unleashing Urease Protein Dynamics with Metamaterial Optical Tweezers |
| topic | Optics Biological Physics |
| url | https://arxiv.org/abs/2402.12878 |