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| Autori principali: | , , , , , , , |
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| Natura: | Preprint |
| Pubblicazione: |
2024
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| Soggetti: | |
| Accesso online: | https://arxiv.org/abs/2402.17964 |
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| _version_ | 1866917599931006976 |
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| author | Kazi, Zeeshawn Shelby, Isaac M. Nirodi, Ruhee Turnbull, Joseph Watanabe, Hideyuki Itoh, Kohei M. Wiggins, Paul A. Fu, Kai-Mei C. |
| author_facet | Kazi, Zeeshawn Shelby, Isaac M. Nirodi, Ruhee Turnbull, Joseph Watanabe, Hideyuki Itoh, Kohei M. Wiggins, Paul A. Fu, Kai-Mei C. |
| contents | DNA flexibility is a key determinant of biological function, from nucleosome positioning to transcriptional regulation, motivating a direct measurement of the bend-torque response of individual DNA molecules. In this work, DNA bending is detected using a nano-mechanical torque balance formed by tethering a ferromagnetic nanoparticle probe by an individual DNA molecule to a diamond magnetic field imager. The torque exerted by the DNA in response to bending caused by an applied magnetic torque is measured using wide-field imaging of quantum defects near the surface of the diamond. Qualitative measurements of differences in DNA bio-mechanical binding configuration are demonstrated, and as a proof-of-principle, a quantitative measurement of the bend response is made for individual DNA molecules. This quantum-enabled measurement approach could be applied to characterize the bend response of biophysically relevant short DNA molecules as well as the sequence dependence of DNA bending energy. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2402_17964 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | Direct measure of DNA bending by quantum magnetic imaging of a nano-mechanical torque-balance Kazi, Zeeshawn Shelby, Isaac M. Nirodi, Ruhee Turnbull, Joseph Watanabe, Hideyuki Itoh, Kohei M. Wiggins, Paul A. Fu, Kai-Mei C. Biological Physics Quantum Physics DNA flexibility is a key determinant of biological function, from nucleosome positioning to transcriptional regulation, motivating a direct measurement of the bend-torque response of individual DNA molecules. In this work, DNA bending is detected using a nano-mechanical torque balance formed by tethering a ferromagnetic nanoparticle probe by an individual DNA molecule to a diamond magnetic field imager. The torque exerted by the DNA in response to bending caused by an applied magnetic torque is measured using wide-field imaging of quantum defects near the surface of the diamond. Qualitative measurements of differences in DNA bio-mechanical binding configuration are demonstrated, and as a proof-of-principle, a quantitative measurement of the bend response is made for individual DNA molecules. This quantum-enabled measurement approach could be applied to characterize the bend response of biophysically relevant short DNA molecules as well as the sequence dependence of DNA bending energy. |
| title | Direct measure of DNA bending by quantum magnetic imaging of a nano-mechanical torque-balance |
| topic | Biological Physics Quantum Physics |
| url | https://arxiv.org/abs/2402.17964 |