3D Super-resolution Optical Fluctuation Imaging with Temporal Focusing two-photon excitation
Fuente:
arXiv
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| Autori principali: | , , |
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| Natura: | Preprint |
| Pubblicazione: |
2024
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| _version_ | 1866917601052983296 |
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| author | Szczypkowski, Pawel Pawlowska, Monika Lapkiewicz, Radek |
| author_facet | Szczypkowski, Pawel Pawlowska, Monika Lapkiewicz, Radek |
| contents | 3D super-resolution fluorescence microscopy typically requires sophisticated setups, sample preparation, or long measurements. A notable exception, SOFI, only requires recording a sequence of frames and no hardware modifications whatsoever but being a wide-field method, it faces problems in thick, dense samples. We combine SOFI with temporal focusing two-photon excitation -- the wide-field method that is capable of excitation of a thin slice in 3D volume. Both methods are easy to implement in a standard microscope, and by merging them, we obtain super-resolved 3D images of neurons stained with quantum dots. Our approach offers reduced bleaching and an improved signal-to-background ratio that can be used when robust resolution improvement is required in thick, dense samples. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2402_19338 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | 3D Super-resolution Optical Fluctuation Imaging with Temporal Focusing two-photon excitation Szczypkowski, Pawel Pawlowska, Monika Lapkiewicz, Radek Optics Biological Physics 3D super-resolution fluorescence microscopy typically requires sophisticated setups, sample preparation, or long measurements. A notable exception, SOFI, only requires recording a sequence of frames and no hardware modifications whatsoever but being a wide-field method, it faces problems in thick, dense samples. We combine SOFI with temporal focusing two-photon excitation -- the wide-field method that is capable of excitation of a thin slice in 3D volume. Both methods are easy to implement in a standard microscope, and by merging them, we obtain super-resolved 3D images of neurons stained with quantum dots. Our approach offers reduced bleaching and an improved signal-to-background ratio that can be used when robust resolution improvement is required in thick, dense samples. |
| title | 3D Super-resolution Optical Fluctuation Imaging with Temporal Focusing two-photon excitation |
| topic | Optics Biological Physics |
| url | https://arxiv.org/abs/2402.19338 |