High throughput spectroscopy of pL droplets
Fuente:
arXiv
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| Autori principali: | , , |
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| Natura: | Preprint |
| Pubblicazione: |
2024
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| _version_ | 1866913256627503104 |
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| author | Sulliger, Marc Arroyo, Jaime Ortega Quidant, Romain |
| author_facet | Sulliger, Marc Arroyo, Jaime Ortega Quidant, Romain |
| contents | Droplet microfluidics offers a versatile platform for analyzing liquid samples. Despite its potential, there is a lack of techniques that allow to reliably probe individual circulating droplets. The prospect of combining droplet microfluidics with sensitive, broadband spectroscopic techniques would therefore unlock new capabilities for various disciplines, including biomedicine and biochemistry. Here we present an integrated optofluidic platform that seamlessly combines droplet microfluidics and advanced hyperspectral imaging. This enables high-resolution, label-free analysis of single picolitre-sized droplets, providing valuable insights into their content. As a proof-of-principle, we demonstrate the ability of our platform to study rapid dynamic changes in a heterogeneous population of plasmonic nanoparticles with millisecond-time resolution. Furthermore, we demonstrate the effectiveness of the platform in biosensing applied to short DNA strands, achieving a detection sensitivity in the range of 100 pM. Finally, we show that the platform provides the flexibility to monitor samples over extended periods of time (hours) in a multiplexed manner. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2403_04027 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | High throughput spectroscopy of pL droplets Sulliger, Marc Arroyo, Jaime Ortega Quidant, Romain Quantitative Methods Biological Physics Droplet microfluidics offers a versatile platform for analyzing liquid samples. Despite its potential, there is a lack of techniques that allow to reliably probe individual circulating droplets. The prospect of combining droplet microfluidics with sensitive, broadband spectroscopic techniques would therefore unlock new capabilities for various disciplines, including biomedicine and biochemistry. Here we present an integrated optofluidic platform that seamlessly combines droplet microfluidics and advanced hyperspectral imaging. This enables high-resolution, label-free analysis of single picolitre-sized droplets, providing valuable insights into their content. As a proof-of-principle, we demonstrate the ability of our platform to study rapid dynamic changes in a heterogeneous population of plasmonic nanoparticles with millisecond-time resolution. Furthermore, we demonstrate the effectiveness of the platform in biosensing applied to short DNA strands, achieving a detection sensitivity in the range of 100 pM. Finally, we show that the platform provides the flexibility to monitor samples over extended periods of time (hours) in a multiplexed manner. |
| title | High throughput spectroscopy of pL droplets |
| topic | Quantitative Methods Biological Physics |
| url | https://arxiv.org/abs/2403.04027 |