Photon Absorption Remote Sensing (PARS): A Comprehensive Approach to Label-free Absorption Microscopy Across Biological Scales
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arXiv
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| Auteurs principaux: | , , , , , |
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| Format: | Preprint |
| Publié: |
2024
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| _version_ | 1866911853632815104 |
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| author | Ecclestone, Benjamin R. Simmons, James A. Tummon Tweel, James E. D. Kaur, Channprit Hajiahmadi, Aria Reza, Parsin Haji |
| author_facet | Ecclestone, Benjamin R. Simmons, James A. Tummon Tweel, James E. D. Kaur, Channprit Hajiahmadi, Aria Reza, Parsin Haji |
| contents | Label-free optical absorption microscopy techniques have evolved as effective tools for non-invasive chemical specific structural, and functional imaging. Yet most modern label-free microscopy modalities target only a fraction of the contrast afforded by an optical absorption interaction. We introduce a comprehensive optical absorption microscopy technique, Photon Absorption Remote Sensing (PARS), which simultaneously captures the dominant light matter interactions which occur as a pulse of light is absorbed by a molecule. In PARS, the optical scattering, attenuation, and the transient radiative and non-radiative relaxation processes are collected at each optical absorption event. This provides a complete representation of the absorption event, providing unique contrast presented here as the total absorption (TA) and quantum efficiency ratio (QER) measurements. By capturing a complete view of each absorption interaction, PARS bridges many of the specificity challenges associated with label-free imaging, facilitating recovery of a wider range of biomolecules than independent radiative or non-radiative modalities. To show the versatility of PARS, we explore imaging across a wide range of biological specimens, from single cells to in-vivo imaging of living subjects. These examples of label-free histopathological imaging, and vascular imaging illustrate some of the numerous fields where PARS may have profound impacts. Overall PARS may provide comprehensive label-free contrast in a wide variety of biological specimens, providing otherwise inaccessible visualizations, and representing a new a source of rich data to develop new AI and machine learning methods for diagnostics and visualization. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2403_04229 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | Photon Absorption Remote Sensing (PARS): A Comprehensive Approach to Label-free Absorption Microscopy Across Biological Scales Ecclestone, Benjamin R. Simmons, James A. Tummon Tweel, James E. D. Kaur, Channprit Hajiahmadi, Aria Reza, Parsin Haji Optics Medical Physics Label-free optical absorption microscopy techniques have evolved as effective tools for non-invasive chemical specific structural, and functional imaging. Yet most modern label-free microscopy modalities target only a fraction of the contrast afforded by an optical absorption interaction. We introduce a comprehensive optical absorption microscopy technique, Photon Absorption Remote Sensing (PARS), which simultaneously captures the dominant light matter interactions which occur as a pulse of light is absorbed by a molecule. In PARS, the optical scattering, attenuation, and the transient radiative and non-radiative relaxation processes are collected at each optical absorption event. This provides a complete representation of the absorption event, providing unique contrast presented here as the total absorption (TA) and quantum efficiency ratio (QER) measurements. By capturing a complete view of each absorption interaction, PARS bridges many of the specificity challenges associated with label-free imaging, facilitating recovery of a wider range of biomolecules than independent radiative or non-radiative modalities. To show the versatility of PARS, we explore imaging across a wide range of biological specimens, from single cells to in-vivo imaging of living subjects. These examples of label-free histopathological imaging, and vascular imaging illustrate some of the numerous fields where PARS may have profound impacts. Overall PARS may provide comprehensive label-free contrast in a wide variety of biological specimens, providing otherwise inaccessible visualizations, and representing a new a source of rich data to develop new AI and machine learning methods for diagnostics and visualization. |
| title | Photon Absorption Remote Sensing (PARS): A Comprehensive Approach to Label-free Absorption Microscopy Across Biological Scales |
| topic | Optics Medical Physics |
| url | https://arxiv.org/abs/2403.04229 |