The role of fluid friction in streamer formation and biofilm growth

Fuente: arXiv
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Main Authors: Wittig, Cornelius, Wagner, Michael, Vallon, Romain, Crouzier, Thomas, van der Wijngaart, Wouter, Horn, Harald, Bagheri, Shervin
Format: Preprint
Published: 2024
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author Wittig, Cornelius
Wagner, Michael
Vallon, Romain
Crouzier, Thomas
van der Wijngaart, Wouter
Horn, Harald
Bagheri, Shervin
author_facet Wittig, Cornelius
Wagner, Michael
Vallon, Romain
Crouzier, Thomas
van der Wijngaart, Wouter
Horn, Harald
Bagheri, Shervin
contents Bacillus subtilis biofilms were grown in laminar channel flow at wall shear stress spanning one order of magnitude ($τ_w = 0.068$ Pa to $τ_w = 0.67$ Pa). We monitor, non-invasively, the evolution of the three-dimensional distribution of biofilm over seven days using optical coherence tomography (OCT). The obtained biofilms consist of many microcolonies where the characteristic colony has a base structure in the form of a leaning pillar and a streamer in the form of a thin filament that originates near the tip of the pillar. While the shape, size and distribution of these microcolonies depend on the imposed shear stress, the same structural features appear consistently for all shear stress values. The formation of streamers seems to occur after the development of a base structure, suggesting that the latter induces a curved secondary flow that triggers the formation of the streamers. Moreover, we observe that the biofilm volume grows approximately linearly over seven days for all the shear stress values, with a growth rate that is inversely proportional to the wall shear stress. We develop a simple model of friction-limited growth, which agrees with the experimental observations. The model provides physical insight into growth mechanisms and can be used to develop accurate continuum models of bacterial biofilm growth.
format Preprint
id arxiv_https___arxiv_org_abs_2403_10545
institution arXiv
publishDate 2024
record_format arxiv
spellingShingle The role of fluid friction in streamer formation and biofilm growth
Wittig, Cornelius
Wagner, Michael
Vallon, Romain
Crouzier, Thomas
van der Wijngaart, Wouter
Horn, Harald
Bagheri, Shervin
Soft Condensed Matter
Fluid Dynamics
Bacillus subtilis biofilms were grown in laminar channel flow at wall shear stress spanning one order of magnitude ($τ_w = 0.068$ Pa to $τ_w = 0.67$ Pa). We monitor, non-invasively, the evolution of the three-dimensional distribution of biofilm over seven days using optical coherence tomography (OCT). The obtained biofilms consist of many microcolonies where the characteristic colony has a base structure in the form of a leaning pillar and a streamer in the form of a thin filament that originates near the tip of the pillar. While the shape, size and distribution of these microcolonies depend on the imposed shear stress, the same structural features appear consistently for all shear stress values. The formation of streamers seems to occur after the development of a base structure, suggesting that the latter induces a curved secondary flow that triggers the formation of the streamers. Moreover, we observe that the biofilm volume grows approximately linearly over seven days for all the shear stress values, with a growth rate that is inversely proportional to the wall shear stress. We develop a simple model of friction-limited growth, which agrees with the experimental observations. The model provides physical insight into growth mechanisms and can be used to develop accurate continuum models of bacterial biofilm growth.
title The role of fluid friction in streamer formation and biofilm growth
topic Soft Condensed Matter
Fluid Dynamics
url https://arxiv.org/abs/2403.10545