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| Main Authors: | , , , , |
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| Format: | Preprint |
| Published: |
2024
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| Subjects: | |
| Online Access: | https://arxiv.org/abs/2404.00824 |
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Table of Contents:
- DNA replication stands as one of the fundamental biological processes crucial for cellular functioning. Recent experimental developments enable the study of replication dynamics at the single-molecule level for complete genomes, facilitating a deeper understanding of its main parameters. In these new data, replication dynamics is reported by the incorporation of an exogenous chemical, whose intra-cellular concentration follows a nonlinear function. The analysis of replication traces thus gives rise to a nonlinear inverse problem, presenting a nonconvex optimization challenge. We demonstrate that under noiseless conditions, the replication dynamics can be uniquely identified by the proposed model. Computing a global solution to this optimization problem is specially challenging because of its multiple local minima. We present the DNA-inverse optimization method that is capable of finding this global solution even in the presence of noise. Comparative analysis against state-of-the-art optimization methods highlights the superior computational efficiency of our approach. DNA-inverse enables the automatic recovery of all configurations of the replication dynamics, which was not possible with previous methods.