SCN as a Local Probe of Protein Structural Dynamics
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arXiv
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| Auteurs principaux: | , , , |
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| Format: | Preprint |
| Publié: |
2024
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| _version_ | 1866916228256235520 |
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| author | Aydin, Sena Salehi, Seyedeh Maryam Töpfer, Kai Meuwly, Markus |
| author_facet | Aydin, Sena Salehi, Seyedeh Maryam Töpfer, Kai Meuwly, Markus |
| contents | The dynamics of lysozyme is probed by attaching -SCN to all alanine-residues. The 1-dimensional infrared spectra exhibit frequency shifts in the position of the maximum absorption by 4 cm$^{-1}$ which is consistent with experiments in different solvents and indicates moderately strong interactions of the vibrational probe with its environment. Isotopic substitution $^{12}$C $\rightarrow ^{13}$C leads to a red-shift by $-47$ cm$^{-1}$ which is consistent with experiments with results on CN-substituted copper complexes in solution. The low-frequency, far-infrared part of the protein spectra contain label-specific information in the difference spectra when compared with the wild type protein. Depending on the positioning of the labels, local structural changes are observed. For example, introducing the -SCN label at Ala129 leads to breaking of the $α-$helical structure with concomitant change in the far-infrared spectrum. Finally, changes in the local hydration of SCN-labelled Alanine residues as a function of time can be related to angular reorientation of the label. It is concluded that -SCN is potentially useful for probing protein dynamics, both in the high-frequency (CN-stretch) and far-infrared part of the spectrum. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2404_18879 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | SCN as a Local Probe of Protein Structural Dynamics Aydin, Sena Salehi, Seyedeh Maryam Töpfer, Kai Meuwly, Markus Chemical Physics The dynamics of lysozyme is probed by attaching -SCN to all alanine-residues. The 1-dimensional infrared spectra exhibit frequency shifts in the position of the maximum absorption by 4 cm$^{-1}$ which is consistent with experiments in different solvents and indicates moderately strong interactions of the vibrational probe with its environment. Isotopic substitution $^{12}$C $\rightarrow ^{13}$C leads to a red-shift by $-47$ cm$^{-1}$ which is consistent with experiments with results on CN-substituted copper complexes in solution. The low-frequency, far-infrared part of the protein spectra contain label-specific information in the difference spectra when compared with the wild type protein. Depending on the positioning of the labels, local structural changes are observed. For example, introducing the -SCN label at Ala129 leads to breaking of the $α-$helical structure with concomitant change in the far-infrared spectrum. Finally, changes in the local hydration of SCN-labelled Alanine residues as a function of time can be related to angular reorientation of the label. It is concluded that -SCN is potentially useful for probing protein dynamics, both in the high-frequency (CN-stretch) and far-infrared part of the spectrum. |
| title | SCN as a Local Probe of Protein Structural Dynamics |
| topic | Chemical Physics |
| url | https://arxiv.org/abs/2404.18879 |